Use of viral entry assays and molecular docking analysis for the identification of antiviral candidates against coxsackievirus a16

Jonathan Y. Wang, Chien Ju Lin, Ching Hsuan Liu, Liang Tzung Lin

Research output: Contribution to journalArticlepeer-review

Abstract

Antiviral assays that mechanistically examine viral entry are pertinent to discern at which step the evaluated agents are most effective, and allow for the identification of candidate viral entry inhibitors. Here, we present the experimental approaches for the identification of small molecules capable of blocking infection by the non-enveloped coxsackievirus A16 (CVA16) through targeting the virus particles or specific steps in early viral entry. Assays include the time-of-drug-addition analysis, flow cytometry-based viral binding assay, and viral inactivation assay. We also present a molecular docking protocol utilizing virus capsid proteins to predict potential residues targeted by the antiviral compounds. These assays should help in the identification of candidate antiviral agents that act on viral entry. Future directions can explore these possible inhibitors for further drug development.

Original languageEnglish
Article numbere59920
JournalJournal of Visualized Experiments
Volume2019
Issue number149
DOIs
Publication statusPublished - Jul 2019

Keywords

  • Antivirals
  • Autodock
  • Binding analysis
  • Drug development
  • Entry inhibitors
  • Immunology and Infection
  • Issue 149
  • Molecular docking
  • PyMol
  • UCSF Chimera
  • Viral entry

ASJC Scopus subject areas

  • Neuroscience(all)
  • Chemical Engineering(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

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