Use of UHPLC-TripleQ with synthetic standards to profile anti-inflammatory hydroxycinnamic acid amides in root barks and leaves of Lycium barbarum

Siyu Wang, Joon Hyuk Suh, Wei Lun Hung, Xi Zheng, Yu Wang, Chi Tang Ho

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Hydroxycinnamic acid amides (HCAA) are the secondary metabolites ubiquitously exist in flowering plants, formed by condensation between hydroxycinnamates and mono or polyamines. HCAA species not only serve multiple functions in plant growth and development, but also exert significant positive effects on human health. In this study, we combined organic synthesis and UPHLC-TripleQ-MS/MS specifically targeting at HCAA species. The method was fully validated with respect to specificity, linearity, intra- and inter-day precision and accuracy, limit of detection (LOD), limit of quantification (LOQ), recovery, and reproducibility. We applied this method to identify and quantify HCAAs from the root barks and leaves of Lycium barbarum. HCAA species were reported in leaves for the first time, and 10 new HCAA species were further identified in root barks in addition to the ones reported in the literature. We also examine anti-inflammatory properties of identified HCAAs species. Seven HCAA compounds had a potent NO inhibitory effect with IC50 as low as 2.381 μM (trans-N-caffeoyl phenethylamine). Our developed method largely improved analytical sensitivity of HCAAs species that potentially contributes to plant metabolomics studies.

Original languageEnglish
Pages (from-to)572-582
Number of pages11
JournalJournal of Food and Drug Analysis
Volume26
Issue number2
DOIs
Publication statusPublished - Apr 1 2018
Externally publishedYes

Fingerprint

Lycium
Lycium barbarum
Coumaric Acids
coumaric acids
amides
Amides
bark
Anti-Inflammatory Agents
leaves
detection limit
phenethylamine
Synthetic Chemistry Techniques
Metabolomics
Plant Development
Polyamines
metabolomics
polyamines
anti-inflammatory activity
Growth and Development
reproducibility

Keywords

  • Anti-inflammatory
  • Hydroxycinnamic acid amide
  • Lycium barbarum
  • Quantification
  • UHPLC-MS/MS

ASJC Scopus subject areas

  • Food Science
  • Pharmacology

Cite this

Use of UHPLC-TripleQ with synthetic standards to profile anti-inflammatory hydroxycinnamic acid amides in root barks and leaves of Lycium barbarum. / Wang, Siyu; Suh, Joon Hyuk; Hung, Wei Lun; Zheng, Xi; Wang, Yu; Ho, Chi Tang.

In: Journal of Food and Drug Analysis, Vol. 26, No. 2, 01.04.2018, p. 572-582.

Research output: Contribution to journalArticle

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