Use of Southern Hybridization to Detect the Haemophilus Influenzae β-lactamase Gene

Hsiu-Chen Lin, Chih Chien Wang, Cheing Mei Yu, Mong Ling Chu

Research output: Contribution to journalArticle

Abstract

 The most common cause of ampicillin resistance in Haemophilus influenzae is due to the production of β-lactamase. In most strains, the β-lactamase produced is TEM-1. Since 1981, ROB-1 β-lactamase appears to be responsible for ampicillin resistance in H. influenzae and several animal pathogens. Forty-five ampicillin-resistant, β-lactamase-producing clinical isolates of Haemophilus influenzae were collected. We used Southern hybridization to determine whether they produced TEM-1 or ROB-1 β-lactamase. Plasmids were obtained from a ROB-1-producing strain of H. influenzae, and a plasmid (pBR322) known to encode TEM-1. Both β-lactamase genes were labeled with non-isotope digoxigenin as probes, and then hybridized on with PCR amplified β-lactamase gene product from H. influenzae strains. The results revealed that β-lactamase-mediated ampicillin resistance type in H. influenzae could be detected by the Southern hybridization method.
Original languageEnglish
Pages (from-to)16-20
Number of pages5
Journal中華民國感染症醫學會雜誌
Volume8
Issue number1
Publication statusPublished - Jun 1997

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Haemophilus influenzae
Southern blotting
ampicillin
genes
plasmids
animal pathogens
digoxigenin

Keywords

  • Southern hybridization
  • β-lactamase
  • Haemophilus influenzae

Cite this

Use of Southern Hybridization to Detect the Haemophilus Influenzae β-lactamase Gene. / Lin, Hsiu-Chen; Wang, Chih Chien; Yu, Cheing Mei; Chu, Mong Ling.

In: 中華民國感染症醫學會雜誌, Vol. 8, No. 1, 06.1997, p. 16-20.

Research output: Contribution to journalArticle

Lin, Hsiu-Chen ; Wang, Chih Chien ; Yu, Cheing Mei ; Chu, Mong Ling. / Use of Southern Hybridization to Detect the Haemophilus Influenzae β-lactamase Gene. In: 中華民國感染症醫學會雜誌. 1997 ; Vol. 8, No. 1. pp. 16-20.
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