Abstract

Background: Current treatments for osteoporosis are associated with various side effects and do not prevent the age-related decrease in osteoblast number. The objective of this study was to evaluate the effects of iQPR-H2O on osteogenesis. Methods. Mouse fibroblast NIH3T3 and pre-osteoblastic MC3T3-E1 cells were cultured in medium prepared with iQPR-H2O or unprocessed mineral water (control cells), and proliferation and differentiation were assessed by MTT and alkaline phosphatase assay, respectively. Mineral deposition by the cells was determined using Alizarin red S staining. A mouse model of osteoporosis, ovariectomized SAMP8 mice, was used to evaluate the effects of iQPR-H2O on osteogenesis in vivo. Mice were given either iQPR-H 2O or unprocessed mineral water (control group) for four months after which bone mass density (BMD) measurements were made using a bone densitometer and hematoxylin and eosin staining of bone samples. Results: NIH3T3 cells grown in medium prepared with iQPR-H2O exhibited significantly greater proliferation. NIH3T3 and MC3T3-E1 cells demonstrated a significant increase in alkaline phosphatase levels in the iQPR-H2O group. MC3T3-E1 cells showed mineralization at day 28. mRNA expression levels of both osteopontin and runt-related transcription factor 2 in MC3T3-E1 cells were higher in the iQPR-H2O group compared with the control group. After four months, significantly greater bone regeneration was evident in ovariectomized SAMP8 mice administered iQPR-H2O as compared with control group. Conclusions: iQPR-H2O may reduce the symptoms of osteoporosis by improving osteogenesis.

Original languageEnglish
Article number227
JournalBMC Musculoskeletal Disorders
Volume12
DOIs
Publication statusPublished - 2011

Fingerprint

Bone Regeneration
Osteoporosis
Osteogenesis
Mineral Waters
Control Groups
Alkaline Phosphatase
Staining and Labeling
Bone and Bones
Osteopontin
Hematoxylin
Eosine Yellowish-(YS)
Osteoblasts
Bone Density
Minerals
Cell Differentiation
Cultured Cells
Transcription Factors
Fibroblasts
Cell Proliferation
Messenger RNA

Keywords

  • bone mass density
  • mouse fibroblasts
  • osteoporosis
  • Quantum Persistent Reflection
  • senescence-accelerated mice

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine
  • Rheumatology

Cite this

Use of iQPR-H2O for bone regeneration and its potential in the improvement of osteoporosis. / Lee, Chi-Ming; Cheong, Meileng; Hsiao, Wentien; Liu, Henyu; Tsai, Chingyu; Wang, Mingfu; Wu, Chihhsiung; Chang, Kwanghwa; Lam, Gowlin; Deng, Winping.

In: BMC Musculoskeletal Disorders, Vol. 12, 227, 2011.

Research output: Contribution to journalArticle

Lee, Chi-Ming ; Cheong, Meileng ; Hsiao, Wentien ; Liu, Henyu ; Tsai, Chingyu ; Wang, Mingfu ; Wu, Chihhsiung ; Chang, Kwanghwa ; Lam, Gowlin ; Deng, Winping. / Use of iQPR-H2O for bone regeneration and its potential in the improvement of osteoporosis. In: BMC Musculoskeletal Disorders. 2011 ; Vol. 12.
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abstract = "Background: Current treatments for osteoporosis are associated with various side effects and do not prevent the age-related decrease in osteoblast number. The objective of this study was to evaluate the effects of iQPR-H2O on osteogenesis. Methods. Mouse fibroblast NIH3T3 and pre-osteoblastic MC3T3-E1 cells were cultured in medium prepared with iQPR-H2O or unprocessed mineral water (control cells), and proliferation and differentiation were assessed by MTT and alkaline phosphatase assay, respectively. Mineral deposition by the cells was determined using Alizarin red S staining. A mouse model of osteoporosis, ovariectomized SAMP8 mice, was used to evaluate the effects of iQPR-H2O on osteogenesis in vivo. Mice were given either iQPR-H 2O or unprocessed mineral water (control group) for four months after which bone mass density (BMD) measurements were made using a bone densitometer and hematoxylin and eosin staining of bone samples. Results: NIH3T3 cells grown in medium prepared with iQPR-H2O exhibited significantly greater proliferation. NIH3T3 and MC3T3-E1 cells demonstrated a significant increase in alkaline phosphatase levels in the iQPR-H2O group. MC3T3-E1 cells showed mineralization at day 28. mRNA expression levels of both osteopontin and runt-related transcription factor 2 in MC3T3-E1 cells were higher in the iQPR-H2O group compared with the control group. After four months, significantly greater bone regeneration was evident in ovariectomized SAMP8 mice administered iQPR-H2O as compared with control group. Conclusions: iQPR-H2O may reduce the symptoms of osteoporosis by improving osteogenesis.",
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author = "Chi-Ming Lee and Meileng Cheong and Wentien Hsiao and Henyu Liu and Chingyu Tsai and Mingfu Wang and Chihhsiung Wu and Kwanghwa Chang and Gowlin Lam and Winping Deng",
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AU - Lee, Chi-Ming

AU - Cheong, Meileng

AU - Hsiao, Wentien

AU - Liu, Henyu

AU - Tsai, Chingyu

AU - Wang, Mingfu

AU - Wu, Chihhsiung

AU - Chang, Kwanghwa

AU - Lam, Gowlin

AU - Deng, Winping

PY - 2011

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N2 - Background: Current treatments for osteoporosis are associated with various side effects and do not prevent the age-related decrease in osteoblast number. The objective of this study was to evaluate the effects of iQPR-H2O on osteogenesis. Methods. Mouse fibroblast NIH3T3 and pre-osteoblastic MC3T3-E1 cells were cultured in medium prepared with iQPR-H2O or unprocessed mineral water (control cells), and proliferation and differentiation were assessed by MTT and alkaline phosphatase assay, respectively. Mineral deposition by the cells was determined using Alizarin red S staining. A mouse model of osteoporosis, ovariectomized SAMP8 mice, was used to evaluate the effects of iQPR-H2O on osteogenesis in vivo. Mice were given either iQPR-H 2O or unprocessed mineral water (control group) for four months after which bone mass density (BMD) measurements were made using a bone densitometer and hematoxylin and eosin staining of bone samples. Results: NIH3T3 cells grown in medium prepared with iQPR-H2O exhibited significantly greater proliferation. NIH3T3 and MC3T3-E1 cells demonstrated a significant increase in alkaline phosphatase levels in the iQPR-H2O group. MC3T3-E1 cells showed mineralization at day 28. mRNA expression levels of both osteopontin and runt-related transcription factor 2 in MC3T3-E1 cells were higher in the iQPR-H2O group compared with the control group. After four months, significantly greater bone regeneration was evident in ovariectomized SAMP8 mice administered iQPR-H2O as compared with control group. Conclusions: iQPR-H2O may reduce the symptoms of osteoporosis by improving osteogenesis.

AB - Background: Current treatments for osteoporosis are associated with various side effects and do not prevent the age-related decrease in osteoblast number. The objective of this study was to evaluate the effects of iQPR-H2O on osteogenesis. Methods. Mouse fibroblast NIH3T3 and pre-osteoblastic MC3T3-E1 cells were cultured in medium prepared with iQPR-H2O or unprocessed mineral water (control cells), and proliferation and differentiation were assessed by MTT and alkaline phosphatase assay, respectively. Mineral deposition by the cells was determined using Alizarin red S staining. A mouse model of osteoporosis, ovariectomized SAMP8 mice, was used to evaluate the effects of iQPR-H2O on osteogenesis in vivo. Mice were given either iQPR-H 2O or unprocessed mineral water (control group) for four months after which bone mass density (BMD) measurements were made using a bone densitometer and hematoxylin and eosin staining of bone samples. Results: NIH3T3 cells grown in medium prepared with iQPR-H2O exhibited significantly greater proliferation. NIH3T3 and MC3T3-E1 cells demonstrated a significant increase in alkaline phosphatase levels in the iQPR-H2O group. MC3T3-E1 cells showed mineralization at day 28. mRNA expression levels of both osteopontin and runt-related transcription factor 2 in MC3T3-E1 cells were higher in the iQPR-H2O group compared with the control group. After four months, significantly greater bone regeneration was evident in ovariectomized SAMP8 mice administered iQPR-H2O as compared with control group. Conclusions: iQPR-H2O may reduce the symptoms of osteoporosis by improving osteogenesis.

KW - bone mass density

KW - mouse fibroblasts

KW - osteoporosis

KW - Quantum Persistent Reflection

KW - senescence-accelerated mice

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