Use of immunomagnetic reduction for C-reactive protein assay in clinical samples

Chien Hsi Chang; Zhi Xian Lai; Hsiu Li Lin; Che Chuan Yang; Hsin Hsien Chen; Shieh Yueh Yang; Herng Er Horng; Chin Yih Hong; Hong Chang Yang; Hsiu Chen Lin

doi:10.2147/IJN.S31030

Use of immunomagnetic reduction for C-reactive protein assay in clinical samples

Chien Hsi Chang, Zhi Xian Lai, Hsiu Li Lin, Che Chuan Yang, Hsin Hsien Chen, Shieh Yueh Yang, Herng Er Horng, Chin Yih Hong, Hong Chang Yang, Hsiu Chen Lin

Research output: Contribution to journal › Article

15 Citations (Scopus)

Abstract

Background: Magnetic nanoparticles biofunctionalized with antibodies are able to recognize and bind to the corresponding antigens. In this work, anti-C-reactive protein (CRP) antibody was covalently conjugated onto the surface of magnetic nanoparticles to label CRP specifically in serum. Methods: The level of serum CRP was detected by immunomagnetic reduction (IMR) assay, which identifies the changes in the magnetic signal representing the level of interaction between antibody-conjugated magnetic nanoparticles and CRP proteins. To investigate the feasibility of IMR for clinical application, pure CRP solutions and 40 human serum samples were tested for IMR detection of CRP to characterize sensitivity, specificity, and interference. Results: In comparison with the immunoturbidimetry assay, the results of the IMR assay indicated higher sensitivity and had a high correlation with those of the current immunoturbidimetry assay. Conclusion: We have developed a novel and promising way to assay CRP in human serum using immunomagnetic reduction in clinical diagnosis.

Original language	English
Pages (from-to)	4335-4340
Number of pages	6
Journal	International Journal of Nanomedicine
Volume	7
DOIs	https://doi.org/10.2147/IJN.S31030
Publication status	Published - 2012

Keywords

C-reactive protein
Immunomagnetic reduction
Magnetic nanoparticles

ASJC Scopus subject areas

Biophysics
Bioengineering
Biomaterials
Organic Chemistry
Drug Discovery

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Chang, C. H., Lai, Z. X., Lin, H. L., Yang, C. C., Chen, H. H., Yang, S. Y., Horng, H. E., Hong, C. Y., Yang, H. C., & Lin, H. C. (2012). Use of immunomagnetic reduction for C-reactive protein assay in clinical samples. International Journal of Nanomedicine, 7, 4335-4340. https://doi.org/10.2147/IJN.S31030

Use of immunomagnetic reduction for C-reactive protein assay in clinical samples. / Chang, Chien Hsi; Lai, Zhi Xian; Lin, Hsiu Li; Yang, Che Chuan; Chen, Hsin Hsien; Yang, Shieh Yueh; Horng, Herng Er; Hong, Chin Yih; Yang, Hong Chang; Lin, Hsiu Chen.

In: International Journal of Nanomedicine, Vol. 7, 2012, p. 4335-4340.

Research output: Contribution to journal › Article

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title = "Use of immunomagnetic reduction for C-reactive protein assay in clinical samples",

abstract = "Background: Magnetic nanoparticles biofunctionalized with antibodies are able to recognize and bind to the corresponding antigens. In this work, anti-C-reactive protein (CRP) antibody was covalently conjugated onto the surface of magnetic nanoparticles to label CRP specifically in serum. Methods: The level of serum CRP was detected by immunomagnetic reduction (IMR) assay, which identifies the changes in the magnetic signal representing the level of interaction between antibody-conjugated magnetic nanoparticles and CRP proteins. To investigate the feasibility of IMR for clinical application, pure CRP solutions and 40 human serum samples were tested for IMR detection of CRP to characterize sensitivity, specificity, and interference. Results: In comparison with the immunoturbidimetry assay, the results of the IMR assay indicated higher sensitivity and had a high correlation with those of the current immunoturbidimetry assay. Conclusion: We have developed a novel and promising way to assay CRP in human serum using immunomagnetic reduction in clinical diagnosis.",

keywords = "C-reactive protein, Immunomagnetic reduction, Magnetic nanoparticles",

author = "Chang, {Chien Hsi} and Lai, {Zhi Xian} and Lin, {Hsiu Li} and Yang, {Che Chuan} and Chen, {Hsin Hsien} and Yang, {Shieh Yueh} and Horng, {Herng Er} and Hong, {Chin Yih} and Yang, {Hong Chang} and Lin, {Hsiu Chen}",

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AU - Chang, Chien Hsi

AU - Lai, Zhi Xian

AU - Lin, Hsiu Li

AU - Yang, Che Chuan

AU - Chen, Hsin Hsien

AU - Yang, Shieh Yueh

AU - Horng, Herng Er

AU - Hong, Chin Yih

AU - Yang, Hong Chang

AU - Lin, Hsiu Chen

PY - 2012

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AB - Background: Magnetic nanoparticles biofunctionalized with antibodies are able to recognize and bind to the corresponding antigens. In this work, anti-C-reactive protein (CRP) antibody was covalently conjugated onto the surface of magnetic nanoparticles to label CRP specifically in serum. Methods: The level of serum CRP was detected by immunomagnetic reduction (IMR) assay, which identifies the changes in the magnetic signal representing the level of interaction between antibody-conjugated magnetic nanoparticles and CRP proteins. To investigate the feasibility of IMR for clinical application, pure CRP solutions and 40 human serum samples were tested for IMR detection of CRP to characterize sensitivity, specificity, and interference. Results: In comparison with the immunoturbidimetry assay, the results of the IMR assay indicated higher sensitivity and had a high correlation with those of the current immunoturbidimetry assay. Conclusion: We have developed a novel and promising way to assay CRP in human serum using immunomagnetic reduction in clinical diagnosis.

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KW - Immunomagnetic reduction

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