Abstract
Background: Magnetic nanoparticles biofunctionalized with antibodies are able to recognize and bind to the corresponding antigens. In this work, anti-C-reactive protein (CRP) antibody was covalently conjugated onto the surface of magnetic nanoparticles to label CRP specifically in serum. Methods: The level of serum CRP was detected by immunomagnetic reduction (IMR) assay, which identifies the changes in the magnetic signal representing the level of interaction between antibody-conjugated magnetic nanoparticles and CRP proteins. To investigate the feasibility of IMR for clinical application, pure CRP solutions and 40 human serum samples were tested for IMR detection of CRP to characterize sensitivity, specificity, and interference. Results: In comparison with the immunoturbidimetry assay, the results of the IMR assay indicated higher sensitivity and had a high correlation with those of the current immunoturbidimetry assay. Conclusion: We have developed a novel and promising way to assay CRP in human serum using immunomagnetic reduction in clinical diagnosis.
| Original language | English |
|---|---|
| Pages (from-to) | 4335-4340 |
| Number of pages | 6 |
| Journal | International Journal of Nanomedicine |
| Volume | 7 |
| DOIs | |
| Publication status | Published - 2012 |
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Keywords
- C-reactive protein
- Immunomagnetic reduction
- Magnetic nanoparticles
ASJC Scopus subject areas
- Biophysics
- Bioengineering
- Biomaterials
- Organic Chemistry
- Drug Discovery
Cite this
Use of immunomagnetic reduction for C-reactive protein assay in clinical samples. / Chang, Chien Hsi; Lai, Zhi Xian; Lin, Hsiu Li; Yang, Che Chuan; Chen, Hsin Hsien; Yang, Shieh Yueh; Horng, Herng Er; Hong, Chin Yih; Yang, Hong Chang; Lin, Hsiu Chen.
In: International Journal of Nanomedicine, Vol. 7, 2012, p. 4335-4340.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Use of immunomagnetic reduction for C-reactive protein assay in clinical samples
AU - Chang, Chien Hsi
AU - Lai, Zhi Xian
AU - Lin, Hsiu Li
AU - Yang, Che Chuan
AU - Chen, Hsin Hsien
AU - Yang, Shieh Yueh
AU - Horng, Herng Er
AU - Hong, Chin Yih
AU - Yang, Hong Chang
AU - Lin, Hsiu Chen
PY - 2012
Y1 - 2012
N2 - Background: Magnetic nanoparticles biofunctionalized with antibodies are able to recognize and bind to the corresponding antigens. In this work, anti-C-reactive protein (CRP) antibody was covalently conjugated onto the surface of magnetic nanoparticles to label CRP specifically in serum. Methods: The level of serum CRP was detected by immunomagnetic reduction (IMR) assay, which identifies the changes in the magnetic signal representing the level of interaction between antibody-conjugated magnetic nanoparticles and CRP proteins. To investigate the feasibility of IMR for clinical application, pure CRP solutions and 40 human serum samples were tested for IMR detection of CRP to characterize sensitivity, specificity, and interference. Results: In comparison with the immunoturbidimetry assay, the results of the IMR assay indicated higher sensitivity and had a high correlation with those of the current immunoturbidimetry assay. Conclusion: We have developed a novel and promising way to assay CRP in human serum using immunomagnetic reduction in clinical diagnosis.
AB - Background: Magnetic nanoparticles biofunctionalized with antibodies are able to recognize and bind to the corresponding antigens. In this work, anti-C-reactive protein (CRP) antibody was covalently conjugated onto the surface of magnetic nanoparticles to label CRP specifically in serum. Methods: The level of serum CRP was detected by immunomagnetic reduction (IMR) assay, which identifies the changes in the magnetic signal representing the level of interaction between antibody-conjugated magnetic nanoparticles and CRP proteins. To investigate the feasibility of IMR for clinical application, pure CRP solutions and 40 human serum samples were tested for IMR detection of CRP to characterize sensitivity, specificity, and interference. Results: In comparison with the immunoturbidimetry assay, the results of the IMR assay indicated higher sensitivity and had a high correlation with those of the current immunoturbidimetry assay. Conclusion: We have developed a novel and promising way to assay CRP in human serum using immunomagnetic reduction in clinical diagnosis.
KW - C-reactive protein
KW - Immunomagnetic reduction
KW - Magnetic nanoparticles
UR - http://www.scopus.com/inward/record.url?scp=84870387460&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84870387460&partnerID=8YFLogxK
U2 - 10.2147/IJN.S31030
DO - 10.2147/IJN.S31030
M3 - Article
VL - 7
SP - 4335
EP - 4340
JO - International Journal of Nanomedicine
JF - International Journal of Nanomedicine
SN - 1176-9114
ER -