Ursolic acid protects hippocampal neurons against kainate-induced excitotoxicity in rats

Yang Hsin Shih, Yi Chun Chein, Jia Yi Wang, Yu Show Fu

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

Ursolic acid is the major component of extracts of the Chinese herb, Souyang. This study determines whether and how ursolic acid protects against kainate-induced excitotoxicity in rat hippocampus. Primary neuronal cultures of cells isolated from the hippocampi of 7-day-old rats were treated with 150 μM kainate. After 2 h of treatment, free radicals were elevated and mitochondrial membrane potential was reduced significantly, and after 12 h, cell viability was decreased. Pretreatment with 5-15 μM ursolic acid dose-dependently and significantly attenuated the kainate-induced damage as well as alleviating the decrease in mitochondrial membrane potential and suppressing the increase in free radical generation. The results suggest that multiple mechanisms including modulation of AMPA receptor, protection of mitochondria, decrease in free radical generation, and scavenging of free radicals might be involved in ursolic acid protection against kainate-induced cell toxicity.

Original languageEnglish
Pages (from-to)136-140
Number of pages5
JournalNeuroscience Letters
Volume362
Issue number2
DOIs
Publication statusPublished - May 20 2004
Externally publishedYes

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Kainic Acid
Free Radicals
Neurons
Mitochondrial Membrane Potential
Hippocampus
Primary Cell Culture
AMPA Receptors
Cell Survival
Mitochondria
ursolic acid

Keywords

  • Excitotoxicity
  • Free radicals
  • Kainate
  • Mitochondrial membrane potential
  • Ursolic acid

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Ursolic acid protects hippocampal neurons against kainate-induced excitotoxicity in rats. / Shih, Yang Hsin; Chein, Yi Chun; Wang, Jia Yi; Fu, Yu Show.

In: Neuroscience Letters, Vol. 362, No. 2, 20.05.2004, p. 136-140.

Research output: Contribution to journalArticle

Shih, Yang Hsin ; Chein, Yi Chun ; Wang, Jia Yi ; Fu, Yu Show. / Ursolic acid protects hippocampal neurons against kainate-induced excitotoxicity in rats. In: Neuroscience Letters. 2004 ; Vol. 362, No. 2. pp. 136-140.
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