Uropathogenic Escherichia coli causes cortical tubular necrotic cell death and the release of macrophage migration inhibitory factor

Ming-Yuan Hong, Chin-Chung Tseng, Chia-Chang Chuang, Chia-Ling Chen, Yu-Huei Lin, Chia-Yuan Hsieh, Yu-Tzu Chang, Chung Hsi Hsing, Kwang-Yu Chang, Chiou Feng Lin

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The macrophage migration inhibitory factor (MIF), a pro-inflammatory cytokine, is deregulated in acute kidney injury (AKI) through an unknown mechanism. In the present study, we used a previously described mouse model of ascending urinary tract infection in which uropathogenic Escherichia coli (UPEC) were transurethrally inoculated to induce kidney infections. Here, we show that urinary MIF was upregulated during AKI while MIF was abundantly expressed in the renal cortical tubules and that UPEC infection caused a decrease in tubular MIF. Infections with UPEC in vitro caused MIF release in a cell type-dependent manner, which was independent of receptor-mediated internalization, signal transduction, and transcription. Indeed, UPEC infection-induced necrotic cell death in vitro and in vivo correlated with extracellular acidification and processed MIF secretion. These data suggest that MIF is released by necrotic renal cortical tubular cells during UPEC infection.

Original languageEnglish
Pages (from-to)945-952
Number of pages8
JournalCytokine
Volume61
Issue number3
DOIs
Publication statusPublished - Mar 2013

Fingerprint

Uropathogenic Escherichia coli
Macrophage Migration-Inhibitory Factors
Cell death
Escherichia coli
Cell Death
Escherichia coli Infections
Kidney
Acute Kidney Injury
Signal transduction
Acidification
Transcription
Infection
Urinary Tract Infections
Signal Transduction
Cytokines

Keywords

  • AKI
  • Cortical tubular cell
  • MIF
  • Necrotic cell death
  • UPEC

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy
  • Hematology
  • Biochemistry
  • Molecular Biology

Cite this

Uropathogenic Escherichia coli causes cortical tubular necrotic cell death and the release of macrophage migration inhibitory factor. / Hong, Ming-Yuan; Tseng, Chin-Chung; Chuang, Chia-Chang; Chen, Chia-Ling; Lin, Yu-Huei; Hsieh, Chia-Yuan; Chang, Yu-Tzu; Hsing, Chung Hsi; Chang, Kwang-Yu; Lin, Chiou Feng.

In: Cytokine, Vol. 61, No. 3, 03.2013, p. 945-952.

Research output: Contribution to journalArticle

Hong, Ming-Yuan ; Tseng, Chin-Chung ; Chuang, Chia-Chang ; Chen, Chia-Ling ; Lin, Yu-Huei ; Hsieh, Chia-Yuan ; Chang, Yu-Tzu ; Hsing, Chung Hsi ; Chang, Kwang-Yu ; Lin, Chiou Feng. / Uropathogenic Escherichia coli causes cortical tubular necrotic cell death and the release of macrophage migration inhibitory factor. In: Cytokine. 2013 ; Vol. 61, No. 3. pp. 945-952.
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