Urinary d-lactate levels reflect renal function in aristolochic acid-induced nephropathy in mice

Tzu Chuan Huang, Shih Ming Chen, Yi Chieh Li, Jen Ai Lee

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Urinary d-lactate is highly correlated to diabetic nephropathy - a progressive kidney disease in renal glomeruli. In this study, we used a C3H/3e mouse model to investigate the relationship between urinary d-lactate and aristolochic acid nephropathy where the glomerular structure is not affected. The nephropathy was induced using intravenous injections of aristolochic acid at a dosage of 10mg/kg per day for 5days and was characterized biochemically and histologically. The urinary excretions of proteins, N-acetyl-β-d-glucosaminidase and serum creatinine were determined and connected to histological conventional findings. Urinary d-lactate was analyzed using column-switching high-performance liquid chromatography with fluorescence detection. The results showed a remarkable increase of urinary markers, including of urinary proteins and N-acetyl-β-d-glucosaminidase, and the histological examination confirmed a diagnosis of acute tubule necrosis. The ratio of d-lactate to creatinine in the urine of aristolochic acid-treated mice was approximately 36 times greater than that of the mice in the control group (p

Original languageEnglish
Pages (from-to)1100-1106
Number of pages7
JournalBiomedical Chromatography
Volume27
Issue number9
DOIs
Publication statusPublished - Sep 2013

Fingerprint

Lactic Acid
Kidney
Hexosaminidases
Creatinine
Inbred C3H Mouse
Kidney Diseases
Diabetic Nephropathies
High performance liquid chromatography
Intravenous Injections
Proteins
Necrosis
Fluorescence
High Pressure Liquid Chromatography
Urine
Control Groups
aristolochic acid I
Serum

Keywords

  • Aristolochic acid nephropathy
  • d-lactate
  • l-lactate
  • Renal injury
  • Urine

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry
  • Molecular Biology
  • Drug Discovery
  • Pharmacology

Cite this

Urinary d-lactate levels reflect renal function in aristolochic acid-induced nephropathy in mice. / Huang, Tzu Chuan; Chen, Shih Ming; Li, Yi Chieh; Lee, Jen Ai.

In: Biomedical Chromatography, Vol. 27, No. 9, 09.2013, p. 1100-1106.

Research output: Contribution to journalArticle

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