Urinary d-lactate levels reflect renal function in aristolochic acid-induced nephropathy in mice

Tzu Chuan Huang, Shih Ming Chen, Yi Chieh Li, Jen Ai Lee

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Urinary d-lactate is highly correlated to diabetic nephropathy - a progressive kidney disease in renal glomeruli. In this study, we used a C3H/3e mouse model to investigate the relationship between urinary d-lactate and aristolochic acid nephropathy where the glomerular structure is not affected. The nephropathy was induced using intravenous injections of aristolochic acid at a dosage of 10mg/kg per day for 5days and was characterized biochemically and histologically. The urinary excretions of proteins, N-acetyl-β-d-glucosaminidase and serum creatinine were determined and connected to histological conventional findings. Urinary d-lactate was analyzed using column-switching high-performance liquid chromatography with fluorescence detection. The results showed a remarkable increase of urinary markers, including of urinary proteins and N-acetyl-β-d-glucosaminidase, and the histological examination confirmed a diagnosis of acute tubule necrosis. The ratio of d-lactate to creatinine in the urine of aristolochic acid-treated mice was approximately 36 times greater than that of the mice in the control group (p

Original languageEnglish
Pages (from-to)1100-1106
Number of pages7
JournalBiomedical Chromatography
Volume27
Issue number9
DOIs
Publication statusPublished - Sep 2013

Keywords

  • Aristolochic acid nephropathy
  • d-lactate
  • l-lactate
  • Renal injury
  • Urine

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry
  • Molecular Biology
  • Drug Discovery
  • Pharmacology

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