Upregulation of the growth arrest-specific-2 in recurrent colorectal cancers, and its susceptibility to chemotherapy in a model cell system

Chi Jung Huang, Chia Long Lee, Shung Haur Yang, Chih Cheng Chien, Chi Cheng Huang, Ruey Neng Yang, Chun Chao Chang

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Colorectal cancer (CRC) is one of the most common life-threatening malignances worldwide. CRC relapse markedly decreases the 5-year survival of patients following surgery. Aberrant expression of genes involved in pathways regulating the cell cycle, cell proliferation, or cell death are frequently reported in CRC tumorigenesis. We hypothesized that genes involved in CRC relapse might serve as prognostic indicators. We first evaluated the significance of gene sequences in the feces of patients with CRC relapse by consulting a public database. Tumorigenesis of target tissues was tested through tumor cell growth, cell cycle regulation, and chemotherapeutic efficacy. We found a highly significant correlation between CRC relapse and growth arrest-specific 2 (GAS2) gene expression. Based on cell models, the overexpressed GAS2 was associated with cellular growth rate, cell cycle regulation, and with chemotherapeutic sensitivity. Cell division was impaired by treating cells with 2-[4-(7-chloro-2-quinoxalinyloxy)phenoxy]-propionic acid (XK469), even when the cells were overexpressing GAS2. Thus, downregulation of GAS2 expression might control CRC relapse after curative resection. GAS2 could serve as a noninvasive marker from the feces of patients with prediagnosed CRC. Our findings suggest that GAS2 could have potential clinical applications for predicting early CRC relapse after radical resection, and that XK469 might impair tumor cell division by reducing GAS2 expression or blocking its cellular translocation. This will help in selecting the best therapeutic option, 5-fluorouracil in combination with XK469, for patients overexpressing GAS2 in CRC cells. Thus, GAS2 might act as a prognostic biomolecule and potential therapeutic target in patients with CRC relapse.

Original languageEnglish
Pages (from-to)1345-1353
Number of pages9
JournalBiochimica et Biophysica Acta - Molecular Basis of Disease
Volume1862
Issue number7
DOIs
Publication statusPublished - Jul 1 2016

Fingerprint

Colorectal Neoplasms
Up-Regulation
Drug Therapy
Growth
Recurrence
Cell Cycle
Feces
Cell Division
Carcinogenesis
Gene Expression
Fluorouracil
Genes
Neoplasms
Cell Death
Down-Regulation
Cell Proliferation
Databases
Survival
Therapeutics

ASJC Scopus subject areas

  • Molecular Biology
  • Molecular Medicine

Cite this

Upregulation of the growth arrest-specific-2 in recurrent colorectal cancers, and its susceptibility to chemotherapy in a model cell system. / Huang, Chi Jung; Lee, Chia Long; Yang, Shung Haur; Chien, Chih Cheng; Huang, Chi Cheng; Yang, Ruey Neng; Chang, Chun Chao.

In: Biochimica et Biophysica Acta - Molecular Basis of Disease, Vol. 1862, No. 7, 01.07.2016, p. 1345-1353.

Research output: Contribution to journalArticle

Huang, Chi Jung ; Lee, Chia Long ; Yang, Shung Haur ; Chien, Chih Cheng ; Huang, Chi Cheng ; Yang, Ruey Neng ; Chang, Chun Chao. / Upregulation of the growth arrest-specific-2 in recurrent colorectal cancers, and its susceptibility to chemotherapy in a model cell system. In: Biochimica et Biophysica Acta - Molecular Basis of Disease. 2016 ; Vol. 1862, No. 7. pp. 1345-1353.
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