Upregulated thymic stromal lymphopoietin receptor expression in children with asthma

Sheng Chieh Lin, Jian Jhang Huang, Jiu Yao Wang, Hsiao Chi Chuang, Bor Luen Chiang, Yi Ling Ye

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Background: Thymic stromal lymphopoietin (TSLP) plays an important role in pathogenesis in patients with asthma. However, the role of thymic stromal lymphopoietin receptor (TSLPR) and correlation with IL-7Rα and clinical severity in asthmatic or nonasthmatic children remain unclear. We investigated TSLPR and IL-7Rα mRΝΑ levels in asthma and nonasthma and assessed TSLPR expression in children who were sensitive to mites. Materials and methods: We enrolled asthmatic and nonasthmatic children. To minimize the influence of allergy, we also divided participants into following 4 groups: nonallergic and nonasthmatic group (NN) (healthy children), allergic but nonasthmatic group (AN), nonallergic but asthmatic group (NA) and allergic asthmatic group (AA). We drew blood samples to check total IgE, allergen-specific IgE and TSLP and measured the expression of the TSLPR and IL-7Rα genes using reverse-transcription polymerase chain reaction (RT-PCR) and real-time PCR. Asthma symptom score was also recorded. Results: Thymic stromal lymphopoietin and TSLPR levels were found to be significantly higher in asthmatic than in nonasthmatic children. The levels of TSLP were found to be significantly different between AA and NN groups (P <0·05). TSLPR expression in NA and AA groups was found to be significantly higher than in NN group (P <0·05). TSLPR did not differ significantly between NA and AA groups. The TSLPR expression correlated strongly with IL-7Rα and weakly with mite-specific IgE. Clinical asthmatic severity of children was found to exert no influence on TSLPR level. Conclusion: Thymic stromal lymphopoietin receptor might be a significant disease biomarker for asthma. The levels of TSLPR were found to be higher in asthmatic patients than in healthy children, but were found to be not different between allergic and nonallergic asthmatic patients.

Original languageEnglish
Pages (from-to)511-519
Number of pages9
JournalEuropean Journal of Clinical Investigation
Volume46
Issue number6
DOIs
Publication statusPublished - Jun 1 2016

Fingerprint

Asthma
Immunoglobulin E
Mites
thymic stromal lymphopoietin
Allergies
Polymerase chain reaction
Biomarkers
Transcription
Allergens
Reverse Transcription
Real-Time Polymerase Chain Reaction
Hypersensitivity
Blood
Genes
Polymerase Chain Reaction

Keywords

  • Allergy
  • Asthma
  • IL-7Rα
  • Mite-sensitive
  • TSLPR

ASJC Scopus subject areas

  • Medicine(all)
  • Clinical Biochemistry
  • Biochemistry

Cite this

Upregulated thymic stromal lymphopoietin receptor expression in children with asthma. / Lin, Sheng Chieh; Huang, Jian Jhang; Wang, Jiu Yao; Chuang, Hsiao Chi; Chiang, Bor Luen; Ye, Yi Ling.

In: European Journal of Clinical Investigation, Vol. 46, No. 6, 01.06.2016, p. 511-519.

Research output: Contribution to journalArticle

Lin, Sheng Chieh ; Huang, Jian Jhang ; Wang, Jiu Yao ; Chuang, Hsiao Chi ; Chiang, Bor Luen ; Ye, Yi Ling. / Upregulated thymic stromal lymphopoietin receptor expression in children with asthma. In: European Journal of Clinical Investigation. 2016 ; Vol. 46, No. 6. pp. 511-519.
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AU - Lin, Sheng Chieh

AU - Huang, Jian Jhang

AU - Wang, Jiu Yao

AU - Chuang, Hsiao Chi

AU - Chiang, Bor Luen

AU - Ye, Yi Ling

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N2 - Background: Thymic stromal lymphopoietin (TSLP) plays an important role in pathogenesis in patients with asthma. However, the role of thymic stromal lymphopoietin receptor (TSLPR) and correlation with IL-7Rα and clinical severity in asthmatic or nonasthmatic children remain unclear. We investigated TSLPR and IL-7Rα mRΝΑ levels in asthma and nonasthma and assessed TSLPR expression in children who were sensitive to mites. Materials and methods: We enrolled asthmatic and nonasthmatic children. To minimize the influence of allergy, we also divided participants into following 4 groups: nonallergic and nonasthmatic group (NN) (healthy children), allergic but nonasthmatic group (AN), nonallergic but asthmatic group (NA) and allergic asthmatic group (AA). We drew blood samples to check total IgE, allergen-specific IgE and TSLP and measured the expression of the TSLPR and IL-7Rα genes using reverse-transcription polymerase chain reaction (RT-PCR) and real-time PCR. Asthma symptom score was also recorded. Results: Thymic stromal lymphopoietin and TSLPR levels were found to be significantly higher in asthmatic than in nonasthmatic children. The levels of TSLP were found to be significantly different between AA and NN groups (P <0·05). TSLPR expression in NA and AA groups was found to be significantly higher than in NN group (P <0·05). TSLPR did not differ significantly between NA and AA groups. The TSLPR expression correlated strongly with IL-7Rα and weakly with mite-specific IgE. Clinical asthmatic severity of children was found to exert no influence on TSLPR level. Conclusion: Thymic stromal lymphopoietin receptor might be a significant disease biomarker for asthma. The levels of TSLPR were found to be higher in asthmatic patients than in healthy children, but were found to be not different between allergic and nonallergic asthmatic patients.

AB - Background: Thymic stromal lymphopoietin (TSLP) plays an important role in pathogenesis in patients with asthma. However, the role of thymic stromal lymphopoietin receptor (TSLPR) and correlation with IL-7Rα and clinical severity in asthmatic or nonasthmatic children remain unclear. We investigated TSLPR and IL-7Rα mRΝΑ levels in asthma and nonasthma and assessed TSLPR expression in children who were sensitive to mites. Materials and methods: We enrolled asthmatic and nonasthmatic children. To minimize the influence of allergy, we also divided participants into following 4 groups: nonallergic and nonasthmatic group (NN) (healthy children), allergic but nonasthmatic group (AN), nonallergic but asthmatic group (NA) and allergic asthmatic group (AA). We drew blood samples to check total IgE, allergen-specific IgE and TSLP and measured the expression of the TSLPR and IL-7Rα genes using reverse-transcription polymerase chain reaction (RT-PCR) and real-time PCR. Asthma symptom score was also recorded. Results: Thymic stromal lymphopoietin and TSLPR levels were found to be significantly higher in asthmatic than in nonasthmatic children. The levels of TSLP were found to be significantly different between AA and NN groups (P <0·05). TSLPR expression in NA and AA groups was found to be significantly higher than in NN group (P <0·05). TSLPR did not differ significantly between NA and AA groups. The TSLPR expression correlated strongly with IL-7Rα and weakly with mite-specific IgE. Clinical asthmatic severity of children was found to exert no influence on TSLPR level. Conclusion: Thymic stromal lymphopoietin receptor might be a significant disease biomarker for asthma. The levels of TSLPR were found to be higher in asthmatic patients than in healthy children, but were found to be not different between allergic and nonallergic asthmatic patients.

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