Up-regulation of inhibitory natural killer receptors CD94/NKG2A with suppressed intracellular perforin expression of tumor-infiltrating CD8 + T lymphocytes in human cervical carcinoma

Bor Ching Sheu, Shin Heng Chiou, Ho Hsiung Lin, Song Nan Chow, Su Cheng Huang, Hong Nerng Ho, Su Ming Hsu

Research output: Contribution to journalArticle

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Abstract

Inhibitory signals that govern the cytolytic functions of CD8 + T lymphocytes have been linked to the expression of natural killer cell receptors (NKRs) on CTLs. There is limited knowledge about the induction of inhibitory NKR (iNKR) expression in vivo. Up-regulation of iNKRs has been linked to the modulation of the virus- and/or tumor-specific immune responses in animal models. In the present study, we directly examined the expression of various NKRs on tumor-infiltrating lymphocytes (TILs) derived from human cervical cancer. We found that in human cervical cancer, the percentage expression of immunoglobulin-like NKR + CD8 + T lymphocytes were similar in gated CD8 + -autologous TILs and peripheral blood mononuclear cells. On the contrary, cervical cancer-infiltrating CD8 + T lymphocytes expressed up-regulated C-type lectin NKRs CD94/NKG2A compared with either peripheral blood CD8 + T cells or normal cervix-infiltrating CD8 + T lymphocytes. Dual NKR coexpression analyses showed that CD94 and NKG2A were mainly expressed on CD56 - CD161 - CD8 + TILs within the cancer milieu. Immunohistochemical study showed that cervical cancer cells expressed abundant interleukin 15 (IL-15) and transforming growth factor-β (TGF-β). In kinetic coculture assay, cervical cancer cells can promote the expression of CD94/NKG2A on CD8 + T lymphocytes. The cancer-derived effects can be reversed by addition of rIL-15Rα/Fc and anti-TGF-β antibody. Functional analyses illustrated that intracellular perforin expression of CD8 + T cells was minimal upon up-regulation of CD94/NKG2A. Kinetic cytotoxicity assays showed that up-regulated expressions of CD94/NKG2A restrain CD8 + T lymphocyte cytotoxicity. Our study strongly indicated that cervical cancer cells could promote the expression of iNKRs via an IL-15- and possibly TGF-β-mediated mechanism and abrogate the antitumor cytotoxicity of TILs.

Original languageEnglish
Pages (from-to)2921-2929
Number of pages9
JournalCancer Research
Volume65
Issue number7
DOIs
Publication statusPublished - Apr 1 2005
Externally publishedYes

Fingerprint

NK Cell Lectin-Like Receptor Subfamily D
Pore Forming Cytotoxic Proteins
Immunologic Cytotoxicity
Immunologic Receptors
CD Antigens
CD8-Positive T-Lymphocytes
KIR Receptors
C-Type Lectins
Tumor-Infiltrating Lymphocytes
Interleukin-15
Perforin
Mixed Lymphocyte Culture Test
Membrane Glycoproteins
Cytotoxic T-Lymphocytes
Natural Killer Cell Receptors
Recombinant Proteins
Uterine Cervical Neoplasms
Transforming Growth Factor beta
Up-Regulation
Carcinoma

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Up-regulation of inhibitory natural killer receptors CD94/NKG2A with suppressed intracellular perforin expression of tumor-infiltrating CD8 + T lymphocytes in human cervical carcinoma. / Sheu, Bor Ching; Chiou, Shin Heng; Lin, Ho Hsiung; Chow, Song Nan; Huang, Su Cheng; Ho, Hong Nerng; Hsu, Su Ming.

In: Cancer Research, Vol. 65, No. 7, 01.04.2005, p. 2921-2929.

Research output: Contribution to journalArticle

Sheu, Bor Ching ; Chiou, Shin Heng ; Lin, Ho Hsiung ; Chow, Song Nan ; Huang, Su Cheng ; Ho, Hong Nerng ; Hsu, Su Ming. / Up-regulation of inhibitory natural killer receptors CD94/NKG2A with suppressed intracellular perforin expression of tumor-infiltrating CD8 + T lymphocytes in human cervical carcinoma. In: Cancer Research. 2005 ; Vol. 65, No. 7. pp. 2921-2929.
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abstract = "Inhibitory signals that govern the cytolytic functions of CD8 + T lymphocytes have been linked to the expression of natural killer cell receptors (NKRs) on CTLs. There is limited knowledge about the induction of inhibitory NKR (iNKR) expression in vivo. Up-regulation of iNKRs has been linked to the modulation of the virus- and/or tumor-specific immune responses in animal models. In the present study, we directly examined the expression of various NKRs on tumor-infiltrating lymphocytes (TILs) derived from human cervical cancer. We found that in human cervical cancer, the percentage expression of immunoglobulin-like NKR + CD8 + T lymphocytes were similar in gated CD8 + -autologous TILs and peripheral blood mononuclear cells. On the contrary, cervical cancer-infiltrating CD8 + T lymphocytes expressed up-regulated C-type lectin NKRs CD94/NKG2A compared with either peripheral blood CD8 + T cells or normal cervix-infiltrating CD8 + T lymphocytes. Dual NKR coexpression analyses showed that CD94 and NKG2A were mainly expressed on CD56 - CD161 - CD8 + TILs within the cancer milieu. Immunohistochemical study showed that cervical cancer cells expressed abundant interleukin 15 (IL-15) and transforming growth factor-β (TGF-β). In kinetic coculture assay, cervical cancer cells can promote the expression of CD94/NKG2A on CD8 + T lymphocytes. The cancer-derived effects can be reversed by addition of rIL-15Rα/Fc and anti-TGF-β antibody. Functional analyses illustrated that intracellular perforin expression of CD8 + T cells was minimal upon up-regulation of CD94/NKG2A. Kinetic cytotoxicity assays showed that up-regulated expressions of CD94/NKG2A restrain CD8 + T lymphocyte cytotoxicity. Our study strongly indicated that cervical cancer cells could promote the expression of iNKRs via an IL-15- and possibly TGF-β-mediated mechanism and abrogate the antitumor cytotoxicity of TILs.",
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AU - Lin, Ho Hsiung

AU - Chow, Song Nan

AU - Huang, Su Cheng

AU - Ho, Hong Nerng

AU - Hsu, Su Ming

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