Up-Regulation of FOXM1 by E6 Oncoprotein through the MZF1/NKX2-1 Axis Is Required for Human Papillomavirus-Associated Tumorigenesis

Po Ming Chen, Ya Wen Cheng, Yao Chen Wang, Tzu Chin Wu, Chih Yi Chen, Huei Lee

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Purpose: Foxhead box M1 (FOXM1) expression has been shown to be linked with human papillomavirus (HPV) 16/18-infected cervical cancer. However, the mechanism underlying the induction of FOXM1 in HPV 16/18-infected cancers remains elusive. Experimental Design: The mechanistic actions of FOXM1 induced by the E6/NKX2-1 axis in tumor aggressiveness were elucidated in cellular and animal models. The prognostic value of FOXM1 for overall survival (OS) and relapse-free survival (RFS) in HPV-positive oral and lung cancers was assessed using Kaplan-Meier and Cox regression models. Results: Herein, FOXM1 expression is upregulated by E6-mediated NKX2-1 in HPV-positive cervical, oral, and lung cancer cells. Induction of FOXM1 by E6 through the MZF1/NKX2-1 axis is responsible for HPV-mediated soft agar growth, invasiveness, and stemness through activating Wnt/β-catenin signaling pathway. In a nude mice model, metastatic lung tumor nodules in HPV 18 E6-positive GNM or HPV 16 E6-positive TL-1-injected nude mice were markedly decreased in both cell types with E6 knockdown, FOXM1 knockdown, or treatment with FOXM1 inhibitor (thiostrepton). Among the four subgroup patients, the worst FOXM1 prognostic value for OS and RFS was observed in HPV 16/18-positive patients with tumors with high-expressing FOXM1. Conclusions: Induction of FOXM1 by E6 oncoprotein through the MZF1/NKX2-1 axis may be responsible for HPV 16/18-mediated tumor progression and poor outcomes in HPV-positive patients.

Original languageEnglish
Pages (from-to)961-971
Number of pages11
JournalNeoplasia
Volume16
Issue number11
DOIs
Publication statusPublished - 2014

Fingerprint

Human papillomavirus 18
Human papillomavirus 16
Oncogene Proteins
Carcinogenesis
Up-Regulation
Survival
Mouth Neoplasms
Neoplasms
Nude Mice
Uterine Cervical Neoplasms
Lung Neoplasms
Thiostrepton
Recurrence
Catenins
Wnt Signaling Pathway
Proportional Hazards Models
Agar
Research Design
Animal Models
Lung

ASJC Scopus subject areas

  • Cancer Research

Cite this

Up-Regulation of FOXM1 by E6 Oncoprotein through the MZF1/NKX2-1 Axis Is Required for Human Papillomavirus-Associated Tumorigenesis. / Chen, Po Ming; Cheng, Ya Wen; Wang, Yao Chen; Wu, Tzu Chin; Chen, Chih Yi; Lee, Huei.

In: Neoplasia, Vol. 16, No. 11, 2014, p. 961-971.

Research output: Contribution to journalArticle

Chen, Po Ming ; Cheng, Ya Wen ; Wang, Yao Chen ; Wu, Tzu Chin ; Chen, Chih Yi ; Lee, Huei. / Up-Regulation of FOXM1 by E6 Oncoprotein through the MZF1/NKX2-1 Axis Is Required for Human Papillomavirus-Associated Tumorigenesis. In: Neoplasia. 2014 ; Vol. 16, No. 11. pp. 961-971.
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abstract = "Purpose: Foxhead box M1 (FOXM1) expression has been shown to be linked with human papillomavirus (HPV) 16/18-infected cervical cancer. However, the mechanism underlying the induction of FOXM1 in HPV 16/18-infected cancers remains elusive. Experimental Design: The mechanistic actions of FOXM1 induced by the E6/NKX2-1 axis in tumor aggressiveness were elucidated in cellular and animal models. The prognostic value of FOXM1 for overall survival (OS) and relapse-free survival (RFS) in HPV-positive oral and lung cancers was assessed using Kaplan-Meier and Cox regression models. Results: Herein, FOXM1 expression is upregulated by E6-mediated NKX2-1 in HPV-positive cervical, oral, and lung cancer cells. Induction of FOXM1 by E6 through the MZF1/NKX2-1 axis is responsible for HPV-mediated soft agar growth, invasiveness, and stemness through activating Wnt/β-catenin signaling pathway. In a nude mice model, metastatic lung tumor nodules in HPV 18 E6-positive GNM or HPV 16 E6-positive TL-1-injected nude mice were markedly decreased in both cell types with E6 knockdown, FOXM1 knockdown, or treatment with FOXM1 inhibitor (thiostrepton). Among the four subgroup patients, the worst FOXM1 prognostic value for OS and RFS was observed in HPV 16/18-positive patients with tumors with high-expressing FOXM1. Conclusions: Induction of FOXM1 by E6 oncoprotein through the MZF1/NKX2-1 axis may be responsible for HPV 16/18-mediated tumor progression and poor outcomes in HPV-positive patients.",
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AU - Cheng, Ya Wen

AU - Wang, Yao Chen

AU - Wu, Tzu Chin

AU - Chen, Chih Yi

AU - Lee, Huei

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