Unpredictability of Triphenyltetrazolium Chloride in Staining Irreversible Ischaemia-Reperfusion Injury in the Skeletal Muscle of Rats

Hui Teng Cheng, Hang Chang, Horng June Lue, Chen Yuan Wen

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Objective: To find out whether pale staining with triphenyltetrazolium chloride (TTC) in skeletal muscle of rat hindlimbs which had been subjected to ischaemia-reperfusion definitely indicated irreversible tissue damage. Design: Laboratory study. Setting: University hospital, Taiwan. Material: 77 Female Wistar rats. Interventions: Ischaemia of one hindlimb was caused by wrapping of a tourniquet above knee joint for 1.5 (n = 14), 2 (n = 15), 2.5 (n = 17), 3 (n = 17) or 4 (n = 14) hours. Each ischaemic group was divided into three subgroups to receive nil, 1 or 1.5 hours reperfusion, respectively. Gastrocnemius and soleus muscles were excised bilaterally. Main outcome measures: TTC reduction in the ischaemic limbs presented as a percentage of the opposite control limb measured by a spectrophotometric assay. Density of red formazan deposition in the ischaemic limbs assessed by microscopic examination of the TTC histochemical stain in the 3 hour ischaemic group. Results: In the 2, 2.5, and 3 hour ischaemic groups the TTC reduction in the ischaemic limbs after 1.5 hours reperfusion increased significantly as compared with that with one hour reperfusion (p <0.01 in each case). The density of red formazan deposition in the muscle after 3 hours ischaemia and 1.5 hours reperfusion was significantly higher than that of only 1 hour of reperfusion. Conclusion: Lack of TTC staining does not necessarily represent irreversible ischaemia-reperfusion injury of the skeletal muscle in rats.

Original languageEnglish
Pages (from-to)407-412
Number of pages6
JournalEuropean Journal of Surgery, Acta Chirurgica
Volume162
Issue number5
Publication statusPublished - 1996
Externally publishedYes

Fingerprint

Reperfusion Injury
Reperfusion
Skeletal Muscle
Staining and Labeling
Extremities
Formazans
Ischemia
Hindlimb
Tourniquets
Knee Joint
Taiwan
triphenyltetrazolium
Wistar Rats
Coloring Agents
Outcome Assessment (Health Care)
Muscles

Keywords

  • Formazan
  • Hindlimb
  • Necrosis
  • Tetrazolium
  • Wistar rat

ASJC Scopus subject areas

  • Surgery
  • Medicine(all)

Cite this

Unpredictability of Triphenyltetrazolium Chloride in Staining Irreversible Ischaemia-Reperfusion Injury in the Skeletal Muscle of Rats. / Cheng, Hui Teng; Chang, Hang; Lue, Horng June; Wen, Chen Yuan.

In: European Journal of Surgery, Acta Chirurgica, Vol. 162, No. 5, 1996, p. 407-412.

Research output: Contribution to journalArticle

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abstract = "Objective: To find out whether pale staining with triphenyltetrazolium chloride (TTC) in skeletal muscle of rat hindlimbs which had been subjected to ischaemia-reperfusion definitely indicated irreversible tissue damage. Design: Laboratory study. Setting: University hospital, Taiwan. Material: 77 Female Wistar rats. Interventions: Ischaemia of one hindlimb was caused by wrapping of a tourniquet above knee joint for 1.5 (n = 14), 2 (n = 15), 2.5 (n = 17), 3 (n = 17) or 4 (n = 14) hours. Each ischaemic group was divided into three subgroups to receive nil, 1 or 1.5 hours reperfusion, respectively. Gastrocnemius and soleus muscles were excised bilaterally. Main outcome measures: TTC reduction in the ischaemic limbs presented as a percentage of the opposite control limb measured by a spectrophotometric assay. Density of red formazan deposition in the ischaemic limbs assessed by microscopic examination of the TTC histochemical stain in the 3 hour ischaemic group. Results: In the 2, 2.5, and 3 hour ischaemic groups the TTC reduction in the ischaemic limbs after 1.5 hours reperfusion increased significantly as compared with that with one hour reperfusion (p <0.01 in each case). The density of red formazan deposition in the muscle after 3 hours ischaemia and 1.5 hours reperfusion was significantly higher than that of only 1 hour of reperfusion. Conclusion: Lack of TTC staining does not necessarily represent irreversible ischaemia-reperfusion injury of the skeletal muscle in rats.",
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