Ultrastructural localisation of NADPH-d/nNOS expression in the superior cervical ganglion of the hamster

Chi Yu Tseng, June Horng Lue, Hung Ming Chang, Chen Yuan Wen, Jeng Yung Shieh

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

This study examined NADPH-d and nNOS expression in the SCG of hamsters. By light microscopy, numerous NADPH-d/NOS positive processes were widely distributed in the ganglion. Ultrastructurally, the NADPH-d reaction product was associated with the membranous organelles of neuronal soma, dendrites, myelinated fibres, small granular cells, and axon profiles bearing agranular vesicles. The NOS immunoreaction product, on the other hand, was localised in the cytoplasm of principal neurons and dendrites. Some of the NADPH-d/NOS labelled processes formed junctional contrasts including synapses or zonulae adherentia. Compared with the neurons, the nonneuronal cells in the ganglion, namely, macrophages, satellite cells and endothelial cells were labelled by NADPH-d but devoid of nNOS immunoreaction product. The results suggest that the NADPH-d/NOS positive fibres in the SCG originate not only from the projecting fibres of the lateral horns of thoracic spinal cord, but also from the principal neurons and small granular cells; some may represent visceral afferent fibres. Electron microscopic morphometry has shown that about 67% of the principal neurons contain NADPH-d reaction product, and that the majority were small to medium sized neurons based on cross-sectional areas in image analysis. On the basis of the present morphological study, it is concluded NO is produced by some local neurons and possibly some nonneuronal cells in the SCG as well as some fibres of extrinsic origin. In this connection, NO may serve either as a neurotransmitter or neuromodulator.

Original languageEnglish
Pages (from-to)461-475
Number of pages15
JournalJournal of Anatomy
Volume197
Issue number3
DOIs
Publication statusPublished - 2000
Externally publishedYes

Fingerprint

Superior Cervical Ganglion
NADP
hamsters
NADP (coenzyme)
Cricetinae
neurons
Neurons
dendrites
Dendrites
neurotransmitters
Ganglia
Neurotransmitter Agents
cells
morphometry
Visceral Afferents
cytoplasm
vesicle
image analysis
microscopy
Carisoprodol

Keywords

  • Hamster
  • Nitric oxide
  • Superior cervical ganglion

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Anatomy

Cite this

Ultrastructural localisation of NADPH-d/nNOS expression in the superior cervical ganglion of the hamster. / Tseng, Chi Yu; Lue, June Horng; Chang, Hung Ming; Wen, Chen Yuan; Shieh, Jeng Yung.

In: Journal of Anatomy, Vol. 197, No. 3, 2000, p. 461-475.

Research output: Contribution to journalArticle

Tseng, Chi Yu ; Lue, June Horng ; Chang, Hung Ming ; Wen, Chen Yuan ; Shieh, Jeng Yung. / Ultrastructural localisation of NADPH-d/nNOS expression in the superior cervical ganglion of the hamster. In: Journal of Anatomy. 2000 ; Vol. 197, No. 3. pp. 461-475.
@article{f5e741f597a44f63922155db7e7447bb,
title = "Ultrastructural localisation of NADPH-d/nNOS expression in the superior cervical ganglion of the hamster",
abstract = "This study examined NADPH-d and nNOS expression in the SCG of hamsters. By light microscopy, numerous NADPH-d/NOS positive processes were widely distributed in the ganglion. Ultrastructurally, the NADPH-d reaction product was associated with the membranous organelles of neuronal soma, dendrites, myelinated fibres, small granular cells, and axon profiles bearing agranular vesicles. The NOS immunoreaction product, on the other hand, was localised in the cytoplasm of principal neurons and dendrites. Some of the NADPH-d/NOS labelled processes formed junctional contrasts including synapses or zonulae adherentia. Compared with the neurons, the nonneuronal cells in the ganglion, namely, macrophages, satellite cells and endothelial cells were labelled by NADPH-d but devoid of nNOS immunoreaction product. The results suggest that the NADPH-d/NOS positive fibres in the SCG originate not only from the projecting fibres of the lateral horns of thoracic spinal cord, but also from the principal neurons and small granular cells; some may represent visceral afferent fibres. Electron microscopic morphometry has shown that about 67{\%} of the principal neurons contain NADPH-d reaction product, and that the majority were small to medium sized neurons based on cross-sectional areas in image analysis. On the basis of the present morphological study, it is concluded NO is produced by some local neurons and possibly some nonneuronal cells in the SCG as well as some fibres of extrinsic origin. In this connection, NO may serve either as a neurotransmitter or neuromodulator.",
keywords = "Hamster, Nitric oxide, Superior cervical ganglion",
author = "Tseng, {Chi Yu} and Lue, {June Horng} and Chang, {Hung Ming} and Wen, {Chen Yuan} and Shieh, {Jeng Yung}",
year = "2000",
doi = "10.1017/S002187829900672X",
language = "English",
volume = "197",
pages = "461--475",
journal = "Journal of Anatomy",
issn = "0021-8782",
publisher = "Wiley-Blackwell",
number = "3",

}

TY - JOUR

T1 - Ultrastructural localisation of NADPH-d/nNOS expression in the superior cervical ganglion of the hamster

AU - Tseng, Chi Yu

AU - Lue, June Horng

AU - Chang, Hung Ming

AU - Wen, Chen Yuan

AU - Shieh, Jeng Yung

PY - 2000

Y1 - 2000

N2 - This study examined NADPH-d and nNOS expression in the SCG of hamsters. By light microscopy, numerous NADPH-d/NOS positive processes were widely distributed in the ganglion. Ultrastructurally, the NADPH-d reaction product was associated with the membranous organelles of neuronal soma, dendrites, myelinated fibres, small granular cells, and axon profiles bearing agranular vesicles. The NOS immunoreaction product, on the other hand, was localised in the cytoplasm of principal neurons and dendrites. Some of the NADPH-d/NOS labelled processes formed junctional contrasts including synapses or zonulae adherentia. Compared with the neurons, the nonneuronal cells in the ganglion, namely, macrophages, satellite cells and endothelial cells were labelled by NADPH-d but devoid of nNOS immunoreaction product. The results suggest that the NADPH-d/NOS positive fibres in the SCG originate not only from the projecting fibres of the lateral horns of thoracic spinal cord, but also from the principal neurons and small granular cells; some may represent visceral afferent fibres. Electron microscopic morphometry has shown that about 67% of the principal neurons contain NADPH-d reaction product, and that the majority were small to medium sized neurons based on cross-sectional areas in image analysis. On the basis of the present morphological study, it is concluded NO is produced by some local neurons and possibly some nonneuronal cells in the SCG as well as some fibres of extrinsic origin. In this connection, NO may serve either as a neurotransmitter or neuromodulator.

AB - This study examined NADPH-d and nNOS expression in the SCG of hamsters. By light microscopy, numerous NADPH-d/NOS positive processes were widely distributed in the ganglion. Ultrastructurally, the NADPH-d reaction product was associated with the membranous organelles of neuronal soma, dendrites, myelinated fibres, small granular cells, and axon profiles bearing agranular vesicles. The NOS immunoreaction product, on the other hand, was localised in the cytoplasm of principal neurons and dendrites. Some of the NADPH-d/NOS labelled processes formed junctional contrasts including synapses or zonulae adherentia. Compared with the neurons, the nonneuronal cells in the ganglion, namely, macrophages, satellite cells and endothelial cells were labelled by NADPH-d but devoid of nNOS immunoreaction product. The results suggest that the NADPH-d/NOS positive fibres in the SCG originate not only from the projecting fibres of the lateral horns of thoracic spinal cord, but also from the principal neurons and small granular cells; some may represent visceral afferent fibres. Electron microscopic morphometry has shown that about 67% of the principal neurons contain NADPH-d reaction product, and that the majority were small to medium sized neurons based on cross-sectional areas in image analysis. On the basis of the present morphological study, it is concluded NO is produced by some local neurons and possibly some nonneuronal cells in the SCG as well as some fibres of extrinsic origin. In this connection, NO may serve either as a neurotransmitter or neuromodulator.

KW - Hamster

KW - Nitric oxide

KW - Superior cervical ganglion

UR - http://www.scopus.com/inward/record.url?scp=0033653621&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033653621&partnerID=8YFLogxK

U2 - 10.1017/S002187829900672X

DO - 10.1017/S002187829900672X

M3 - Article

C2 - 11117630

AN - SCOPUS:0033653621

VL - 197

SP - 461

EP - 475

JO - Journal of Anatomy

JF - Journal of Anatomy

SN - 0021-8782

IS - 3

ER -