Ultra high-resolution fMRI and electrophysiology of the rat primary somatosensory cortex

Yen-Yu Shih, You-Yin Chen, Hsin-Yi Lai, Yu-Chieh Jill Kao, Bai-Chung Shyu, Timothy Q. Duong

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

High-resolution functional-magnetic-resonance-imaging (fMRI) has been used to study brain functions at increasingly finer scale, but whether fMRI can accurately reflect layer-specific neuronal activities is less well understood. The present study investigated layer-specific cerebral-blood-volume (CBV) fMRI and electrophysiological responses in the rat cortex. CBV fMRI at 40 × 40. μm in-plane resolution was performed on an 11.7-T scanner. Electrophysiology used a 32-channel electrode array that spanned the entire cortical depth. Graded electrical stimulation was used to study activations in different cortical layers, exploiting the notion that most of the sensory-specific neurons are in layers II-V and most of the nociceptive-specific neurons are in layers V-VI. CBV response was strongest in layer IV of all stimulus amplitudes. Current source density analysis showed strong sink currents at cortical layers IV and VI. Multi-unit activities mainly appeared at layers IV-VI and peaked at layer V. Although our measures showed scaled activation profiles during modulation of stimulus amplitude and failed to detect specific recruitment at layers V and VI during noxious electrical stimuli, there appears to be discordance between CBV fMRI and electrophysiological peak responses, suggesting neurovascular uncoupling at laminar resolution. The technique implemented in the present study offers a means to investigate intracortical neurovascular function in the normal and diseased animal models at laminar resolution. © 2013.
Original languageEnglish
Pages (from-to)113-120
Number of pages8
JournalNeuroImage
Volume73
DOIs
Publication statusPublished - 2013
Externally publishedYes

Fingerprint

Somatosensory Cortex
Electrophysiology
Magnetic Resonance Imaging
Animal Disease Models
Nociceptors
Sensory Receptor Cells
Electric Stimulation
Electrodes
Cerebral Blood Volume
Brain

Keywords

  • Cerebral blood volume
  • Current source density
  • FMRI
  • High-resolution
  • Local field potential
  • Rat
  • Somatosensory cortex
  • amplitude modulation
  • animal experiment
  • article
  • brain blood volume
  • brain electrophysiology
  • controlled study
  • electrode
  • electrostimulation
  • female
  • functional magnetic resonance imaging
  • nociceptive stimulation
  • nonhuman
  • primary somatosensory cortex
  • priority journal
  • rat
  • sensory nerve cell
  • stimulus response
  • Animals
  • Data Interpretation, Statistical
  • Electric Stimulation
  • Electromagnetic Fields
  • Electrophysiology
  • Forelimb
  • Magnetic Resonance Imaging
  • Male
  • Rats
  • Rats, Sprague-Dawley
  • Respiration, Artificial
  • Somatosensory Cortex
  • Stereotaxic Techniques

Cite this

Ultra high-resolution fMRI and electrophysiology of the rat primary somatosensory cortex. / Shih, Yen-Yu; Chen, You-Yin; Lai, Hsin-Yi; Kao, Yu-Chieh Jill; Shyu, Bai-Chung; Duong, Timothy Q.

In: NeuroImage, Vol. 73, 2013, p. 113-120.

Research output: Contribution to journalArticle

Shih, Yen-Yu ; Chen, You-Yin ; Lai, Hsin-Yi ; Kao, Yu-Chieh Jill ; Shyu, Bai-Chung ; Duong, Timothy Q. / Ultra high-resolution fMRI and electrophysiology of the rat primary somatosensory cortex. In: NeuroImage. 2013 ; Vol. 73. pp. 113-120.
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abstract = "High-resolution functional-magnetic-resonance-imaging (fMRI) has been used to study brain functions at increasingly finer scale, but whether fMRI can accurately reflect layer-specific neuronal activities is less well understood. The present study investigated layer-specific cerebral-blood-volume (CBV) fMRI and electrophysiological responses in the rat cortex. CBV fMRI at 40 × 40. μm in-plane resolution was performed on an 11.7-T scanner. Electrophysiology used a 32-channel electrode array that spanned the entire cortical depth. Graded electrical stimulation was used to study activations in different cortical layers, exploiting the notion that most of the sensory-specific neurons are in layers II-V and most of the nociceptive-specific neurons are in layers V-VI. CBV response was strongest in layer IV of all stimulus amplitudes. Current source density analysis showed strong sink currents at cortical layers IV and VI. Multi-unit activities mainly appeared at layers IV-VI and peaked at layer V. Although our measures showed scaled activation profiles during modulation of stimulus amplitude and failed to detect specific recruitment at layers V and VI during noxious electrical stimuli, there appears to be discordance between CBV fMRI and electrophysiological peak responses, suggesting neurovascular uncoupling at laminar resolution. The technique implemented in the present study offers a means to investigate intracortical neurovascular function in the normal and diseased animal models at laminar resolution. {\circledC} 2013.",
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author = "Yen-Yu Shih and You-Yin Chen and Hsin-Yi Lai and Kao, {Yu-Chieh Jill} and Bai-Chung Shyu and Duong, {Timothy Q.}",
note = "被引用次數:15 Export Date: 6 April 2016 CODEN: NEIME 通訊地址: Shih, Y.-Y.I.; Experimental Neuroimaging Laboratory, Departments of Neurology and Biomedical Research Imaging Center, University of North Carolina, 130 Mason Farm Road, CB# 7513, Chapel Hill, NC 27599, United States; 電子郵件: shihy@unc.edu 參考文獻: Bannister, A.P., Inter- and intra-laminar connections of pyramidal cells in the neocortex (2005) Neurosci. Res., 53, pp. 95-103; B{\'e}dard, C., Destexhe, A., Generalized theory for current-source-density analysis in brain tissue (2011) Phys. Rev. E, 84, p. 041909; Bernardo, K.L., Woolsey, T.A., Axonal trajectories between mouse somatosensory thalamus and cortex (1987) J. Comp. Neurol., 258, pp. 542-564; Brinker, G., Bock, C., Busch, E., Krep, H., Hossmann, K.A., Hoehn-Berlage, M., Simultaneous recording of evoked potentials and T2*-weighted MR images during somatosensory stimulation of rat (1999) Magn. Reson. 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year = "2013",
doi = "10.1016/j.neuroimage.2013.01.062",
language = "English",
volume = "73",
pages = "113--120",
journal = "NeuroImage",
issn = "1053-8119",
publisher = "Academic Press Inc.",

}

TY - JOUR

T1 - Ultra high-resolution fMRI and electrophysiology of the rat primary somatosensory cortex

AU - Shih, Yen-Yu

AU - Chen, You-Yin

AU - Lai, Hsin-Yi

AU - Kao, Yu-Chieh Jill

AU - Shyu, Bai-Chung

AU - Duong, Timothy Q.

N1 - 被引用次數:15 Export Date: 6 April 2016 CODEN: NEIME 通訊地址: Shih, Y.-Y.I.; Experimental Neuroimaging Laboratory, Departments of Neurology and Biomedical Research Imaging Center, University of North Carolina, 130 Mason Farm Road, CB# 7513, Chapel Hill, NC 27599, United States; 電子郵件: shihy@unc.edu 參考文獻: Bannister, A.P., Inter- and intra-laminar connections of pyramidal cells in the neocortex (2005) Neurosci. Res., 53, pp. 95-103; Bédard, C., Destexhe, A., Generalized theory for current-source-density analysis in brain tissue (2011) Phys. Rev. E, 84, p. 041909; Bernardo, K.L., Woolsey, T.A., Axonal trajectories between mouse somatosensory thalamus and cortex (1987) J. Comp. Neurol., 258, pp. 542-564; Brinker, G., Bock, C., Busch, E., Krep, H., Hossmann, K.A., Hoehn-Berlage, M., Simultaneous recording of evoked potentials and T2*-weighted MR images during somatosensory stimulation of rat (1999) Magn. Reson. 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PY - 2013

Y1 - 2013

N2 - High-resolution functional-magnetic-resonance-imaging (fMRI) has been used to study brain functions at increasingly finer scale, but whether fMRI can accurately reflect layer-specific neuronal activities is less well understood. The present study investigated layer-specific cerebral-blood-volume (CBV) fMRI and electrophysiological responses in the rat cortex. CBV fMRI at 40 × 40. μm in-plane resolution was performed on an 11.7-T scanner. Electrophysiology used a 32-channel electrode array that spanned the entire cortical depth. Graded electrical stimulation was used to study activations in different cortical layers, exploiting the notion that most of the sensory-specific neurons are in layers II-V and most of the nociceptive-specific neurons are in layers V-VI. CBV response was strongest in layer IV of all stimulus amplitudes. Current source density analysis showed strong sink currents at cortical layers IV and VI. Multi-unit activities mainly appeared at layers IV-VI and peaked at layer V. Although our measures showed scaled activation profiles during modulation of stimulus amplitude and failed to detect specific recruitment at layers V and VI during noxious electrical stimuli, there appears to be discordance between CBV fMRI and electrophysiological peak responses, suggesting neurovascular uncoupling at laminar resolution. The technique implemented in the present study offers a means to investigate intracortical neurovascular function in the normal and diseased animal models at laminar resolution. © 2013.

AB - High-resolution functional-magnetic-resonance-imaging (fMRI) has been used to study brain functions at increasingly finer scale, but whether fMRI can accurately reflect layer-specific neuronal activities is less well understood. The present study investigated layer-specific cerebral-blood-volume (CBV) fMRI and electrophysiological responses in the rat cortex. CBV fMRI at 40 × 40. μm in-plane resolution was performed on an 11.7-T scanner. Electrophysiology used a 32-channel electrode array that spanned the entire cortical depth. Graded electrical stimulation was used to study activations in different cortical layers, exploiting the notion that most of the sensory-specific neurons are in layers II-V and most of the nociceptive-specific neurons are in layers V-VI. CBV response was strongest in layer IV of all stimulus amplitudes. Current source density analysis showed strong sink currents at cortical layers IV and VI. Multi-unit activities mainly appeared at layers IV-VI and peaked at layer V. Although our measures showed scaled activation profiles during modulation of stimulus amplitude and failed to detect specific recruitment at layers V and VI during noxious electrical stimuli, there appears to be discordance between CBV fMRI and electrophysiological peak responses, suggesting neurovascular uncoupling at laminar resolution. The technique implemented in the present study offers a means to investigate intracortical neurovascular function in the normal and diseased animal models at laminar resolution. © 2013.

KW - Cerebral blood volume

KW - Current source density

KW - FMRI

KW - High-resolution

KW - Local field potential

KW - Rat

KW - Somatosensory cortex

KW - amplitude modulation

KW - animal experiment

KW - article

KW - brain blood volume

KW - brain electrophysiology

KW - controlled study

KW - electrode

KW - electrostimulation

KW - female

KW - functional magnetic resonance imaging

KW - nociceptive stimulation

KW - nonhuman

KW - primary somatosensory cortex

KW - priority journal

KW - rat

KW - sensory nerve cell

KW - stimulus response

KW - Animals

KW - Data Interpretation, Statistical

KW - Electric Stimulation

KW - Electromagnetic Fields

KW - Electrophysiology

KW - Forelimb

KW - Magnetic Resonance Imaging

KW - Male

KW - Rats

KW - Rats, Sprague-Dawley

KW - Respiration, Artificial

KW - Somatosensory Cortex

KW - Stereotaxic Techniques

U2 - 10.1016/j.neuroimage.2013.01.062

DO - 10.1016/j.neuroimage.2013.01.062

M3 - Article

VL - 73

SP - 113

EP - 120

JO - NeuroImage

JF - NeuroImage

SN - 1053-8119

ER -