Tyrosinase-based TLC Autography for anti-melanogenic drug screening

Kai Di Hsu, Yu Hin Chan, Hong Jhang Chen, Shi Ping Lin, Kuan Chen Cheng

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Tyrosinase-based TLC (thin layer chromatography) was developed for screening of anti-melanogenic drugs. In particular, this technique enables researchers to identify melanogenic inhibitor(s) in tested mixtures with the naked eye. In comparison with traditional colorimetric screening assays for tyrosinase inhibitor(s), not only is tyrosinase-based TLC a more cost-effective option (nearly one-tenth the enzyme cost of colorimetric methods) but also is a more sensitive detection approach for kojic acid (KA), a standard anti-melanogenic drug. The detection limit of tyrosinase-based TLC and colorimetric tyrosinase assay for KA was 0.0125 and 1.25 μg, respectively, demonstrating that the former was 100-fold more sensitive than the latter to determine the tyrosinase inhibitory rate of KA. Furthermore, the results of this method have demonstrated excellent precision by Gage Repeatability and Reproducibility (Gage R&R), with the variation of total Gage R&R being 28.24%. To verify the applicability of tyrosinase-based TLC, this platform was employed to screen melanogenic inhibitor(s) from Ganoderma formosanum extracts and two of all fractions (GFE-EA F4, F5) obtained showed depigmenting activity. It is noteworthy that these two fractions also exerted anti-melanogenesis activity on zebrafish, therefore verifying the credibility of tyrosinase-based TLC. In sum, this technique provides new insight into the discovery of novel melanogenic inhibitor(s).

Original languageEnglish
Article number401
JournalScientific Reports
Volume8
Issue number1
DOIs
Publication statusPublished - Dec 1 2018
Externally publishedYes

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Preclinical Drug Evaluations
Monophenol Monooxygenase
Thin Layer Chromatography
Ganoderma
Costs and Cost Analysis
Zebrafish
Pharmaceutical Preparations
Limit of Detection
Research Personnel
Enzymes

ASJC Scopus subject areas

  • General

Cite this

Tyrosinase-based TLC Autography for anti-melanogenic drug screening. / Hsu, Kai Di; Chan, Yu Hin; Chen, Hong Jhang; Lin, Shi Ping; Cheng, Kuan Chen.

In: Scientific Reports, Vol. 8, No. 1, 401, 01.12.2018.

Research output: Contribution to journalArticle

Hsu, Kai Di ; Chan, Yu Hin ; Chen, Hong Jhang ; Lin, Shi Ping ; Cheng, Kuan Chen. / Tyrosinase-based TLC Autography for anti-melanogenic drug screening. In: Scientific Reports. 2018 ; Vol. 8, No. 1.
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