Type-specific human papillomavirus oncogene messenger RNA levels correlate with the severity of cervical neoplasia

Chih Ming Ho, Bor Heng Lee, Shwu Fen Chang, Tsai Yen Chien, Shih Hung Huang, Chiu Cho Yan, Wen Fang Cheng

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

This study aimed to evaluate whether quantitation of high-risk human papillomavirus (HR-HPV) E6 messenger RNA (mRNA) can be a potential biomarker for detecting the severity of cervical lesions. HPV genotyping was performed using a modified MY11/ GP6+ PCR for HPV DNA amplification, followed by HPV genotype-specific hybridization with on a gene chip. E6 type-specific PCR was used to validate multiple infections. Quantitative real-time reverse transcriptase (QRT-PCR) and real-time PCR used to measure mRNA levels and DNA viral loads of 6 HPV oncogenic types (HPV 16, 18, 31, 33, 52 and 58) in 720 liquid-based cytology samples. The HPV DNA and RNA measurements were correlated with cervical lesions diagnosed by histopathologic examination. mRNA transcripts in the 6 types HPV DNA-positive cases was lower in normal women and

Original languageEnglish
Pages (from-to)622-632
Number of pages11
JournalInternational Journal of Cancer
Volume127
Issue number3
DOIs
Publication statusPublished - Aug 1 2010

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Keywords

  • Cervical intraepithelial neoplasm
  • Human papillomavirus
  • mRNA
  • Viral load

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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