Type-specific human papillomavirus oncogene messenger RNA levels correlate with the severity of cervical neoplasia

Chih Ming Ho, Bor Heng Lee, Shwu Fen Chang, Tsai Yen Chien, Shih Hung Huang, Chiu Cho Yan, Wen Fang Cheng

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

This study aimed to evaluate whether quantitation of high-risk human papillomavirus (HR-HPV) E6 messenger RNA (mRNA) can be a potential biomarker for detecting the severity of cervical lesions. HPV genotyping was performed using a modified MY11/ GP6+ PCR for HPV DNA amplification, followed by HPV genotype-specific hybridization with on a gene chip. E6 type-specific PCR was used to validate multiple infections. Quantitative real-time reverse transcriptase (QRT-PCR) and real-time PCR used to measure mRNA levels and DNA viral loads of 6 HPV oncogenic types (HPV 16, 18, 31, 33, 52 and 58) in 720 liquid-based cytology samples. The HPV DNA and RNA measurements were correlated with cervical lesions diagnosed by histopathologic examination. mRNA transcripts in the 6 types HPV DNA-positive cases was lower in normal women and

Original languageEnglish
Pages (from-to)622-632
Number of pages11
JournalInternational Journal of Cancer
Volume127
Issue number3
DOIs
Publication statusPublished - Aug 1 2010

Fingerprint

Oncogenes
Human papillomavirus 6
Messenger RNA
DNA
Real-Time Polymerase Chain Reaction
Neoplasms
Human papillomavirus 18
Polymerase Chain Reaction
Human papillomavirus 16
Oligonucleotide Array Sequence Analysis
Viral Load
Reverse Transcriptase Polymerase Chain Reaction
Cell Biology
Biomarkers
Genotype
RNA
Infection

Keywords

  • Cervical intraepithelial neoplasm
  • Human papillomavirus
  • mRNA
  • Viral load

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Type-specific human papillomavirus oncogene messenger RNA levels correlate with the severity of cervical neoplasia. / Ho, Chih Ming; Lee, Bor Heng; Chang, Shwu Fen; Chien, Tsai Yen; Huang, Shih Hung; Yan, Chiu Cho; Cheng, Wen Fang.

In: International Journal of Cancer, Vol. 127, No. 3, 01.08.2010, p. 622-632.

Research output: Contribution to journalArticle

Ho, Chih Ming ; Lee, Bor Heng ; Chang, Shwu Fen ; Chien, Tsai Yen ; Huang, Shih Hung ; Yan, Chiu Cho ; Cheng, Wen Fang. / Type-specific human papillomavirus oncogene messenger RNA levels correlate with the severity of cervical neoplasia. In: International Journal of Cancer. 2010 ; Vol. 127, No. 3. pp. 622-632.
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