Type-specific human papillomavirus DNA testing with the genotyping array: A comparison of cervical and vaginal sampling

Nae Fang Twu, Ming Shyen Yen, Hei Yu Lau, Yi Jen Chen, Bill Ken Jen Yu, Ching Yu Lin

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Objective: To compare the detection and typing of human papillomavirus (HPV) between vaginal and cervical specimens by using polymerase chain reaction (PCR)-based reverse-blot genotyping arrays. Study design: Two hundred and fifty-two women were referred to colposcopy clinics because of suspicious or positive results in a community-based cervical cancer-screening program. Genital tract cells were sampled from the cervix and self-collected from the vagina and tested with the HPV Blot kit. Results: The HPV Blot kit identified HPV infection in 24.7% of vaginal specimens and in 30.2% of cervical collections. Cervical sampling detected significantly more infections compared to vaginal sampling only for HPV type 52; cervical sampling also detected significantly more high-risk HPV infection overall. The sensitivities of detecting histology ≥cervical intraepithelial neoplasia (CIN) grade 3 using the HPV Blot in vaginal and cervical specimens were 75.0% (95% CI, 47.6-92.7%) and 87.5% (95% CI, 61.6-98.4%), respectively (P = 0.48). Both sampling methods were thus statistically effective at detecting high-grade lesions and cervical cancer (P <0.0001). Conclusions: The HPV Blot yielded similar results for both vaginal sampling and cervical sampling in the detection of CIN grade 3 or worse. These findings indicate that self-sampling for HPV testing is a viable cervical cancer screening option.

Original languageEnglish
Pages (from-to)96-100
Number of pages5
JournalEuropean Journal of Obstetrics Gynecology and Reproductive Biology
Volume156
Issue number1
DOIs
Publication statusPublished - May 2011

Keywords

  • Cervical cancer
  • Cervical sampling
  • HPV Blot
  • Human papillomavirus
  • Vaginal sampling

ASJC Scopus subject areas

  • Obstetrics and Gynaecology
  • Reproductive Medicine

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