Abstract

To improve the bioactivity of titanium surfaces, glow discharge was used to facilitate collagen grafting on titanium disks. Titanium test specimens were pre-treated by glow discharge fed with a mixture of argon and allylamine (AA) gases. Treated titanium disks were then grafted with type I collagen using glutaraldehyde (GA) as a crosslinking agent. The surfaces of collagen-grafted titanium disks were evaluated using scanning electron microscopy-energy dispersive spectroscopy (SEM-EDS) and X-ray photoelectron spectroscopy (XPS). MG-63 osteoblast-like cells were cultured on the grafted titanium surfaces to examine the effect of collagen grafting in terms of cell morphology. Our results demonstrated that collagen component elements could be detected on the titanium surfaces. Morphology of the cells on the surfaces of collagen-grafted titanium disks indicated differentiation. These findings showed that type I collagen could be successfully grafted onto titanium surfaces using glow discharge technology, with enhanced biofunctionality demonstrated on osteoblastic cells.

Original languageEnglish
Pages (from-to)340-346
Number of pages7
JournalDental Materials Journal
Volume27
Issue number3
DOIs
Publication statusPublished - 2008

Fingerprint

Glow discharges
Collagen Type I
Titanium
Collagen
Temperature
Allylamine
X-Ray Emission Spectrometry
Photoelectron Spectroscopy
Argon
Osteoblasts
Glutaral
Bioactivity
Crosslinking
Electron Scanning Microscopy
Energy dispersive spectroscopy
Cultured Cells
Spectrum Analysis
X ray photoelectron spectroscopy
Gases
Technology

Keywords

  • Collagen
  • Glow discharge
  • Titanium

ASJC Scopus subject areas

  • Dentistry(all)
  • Biomaterials

Cite this

Type I collagen grafting on titanium surfaces using low-temperature glow discharge. / Chang, Wei Jen; Ou, Keng Liang; Lee, Sheng Yang; Chen, Jin Yu; Abiko, Yoshimitsu; Lin, Che Tong; Huang, Haw Ming.

In: Dental Materials Journal, Vol. 27, No. 3, 2008, p. 340-346.

Research output: Contribution to journalArticle

Chang, Wei Jen ; Ou, Keng Liang ; Lee, Sheng Yang ; Chen, Jin Yu ; Abiko, Yoshimitsu ; Lin, Che Tong ; Huang, Haw Ming. / Type I collagen grafting on titanium surfaces using low-temperature glow discharge. In: Dental Materials Journal. 2008 ; Vol. 27, No. 3. pp. 340-346.
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