TY - JOUR
T1 - Type I and II collagen regulation of chondrogenic differentiation by mesenchymal progenitor cells
AU - Chen, C. W.
AU - Tsai, Yu-Hui
AU - Deng, W. P.
AU - Shih, S. N.
AU - Fang, C. L.
AU - Burch, J. G.
AU - Chen, W. H.
AU - Lai, Wen-FuThomas
N1 - Funding Information:
This work was supported by the National Science Council, Taiwan, R.O.C. under project no. NSC91-2314-B-038-033. The authors thank Prof C.Y. Yeh for statistical advice, and Ching-Jin Tu and Ching-Fu Liao for their technical help with histological preparations.
Copyright:
Copyright 2008 Elsevier B.V., All rights reserved.
PY - 2005/3
Y1 - 2005/3
N2 - Chondrogenic differentiation by mesenchymal progenitor cells (MPCs) is associated with cytokines such as transforming growth factor-beta 1 (TGF-β1) and dexamethasone. Extracellular matrix (ECM) also regulates the differentiation by MPCs. To define whether ECM plays a functional role in regulation of the chondrogenic differentiation by MPCs, an in vitro model was used. That model exposed to dexamethasone, recombinant human TGF-β1(rhTGF-β1) and collagens. The results showed that MPCs incorporated with dexamethasone and rhTGF-β1 increased proliferation and expression of glycosaminoglycan (GAG) after 14 days. Type II collagen enhanced the GAG synthesis, but did not increase alkaline phosphatase (ALP) activity. When adding dexamethasone and rhTGF-β1 MPCs increased mRNA expression of Sox9. Incorporation with type II collagen, dexamethasone and rhTGF-β1, MPCs induced mRNA expression of aggrecan and enhanced levels of type II collagen, and Sox9 mRNA. In contrast, incorporation with type I collagen, dexamethasone and rhTGF-β1 MPCs reduced levels of aggrecan, and Sox9 mRNA, and showed no type II collagen mRNA. Altogether, these results indicate that type I and II collagen, in addition to the cytokine effect, may play a functional role in regulating of chondrogenic differentiation by MPCs.
AB - Chondrogenic differentiation by mesenchymal progenitor cells (MPCs) is associated with cytokines such as transforming growth factor-beta 1 (TGF-β1) and dexamethasone. Extracellular matrix (ECM) also regulates the differentiation by MPCs. To define whether ECM plays a functional role in regulation of the chondrogenic differentiation by MPCs, an in vitro model was used. That model exposed to dexamethasone, recombinant human TGF-β1(rhTGF-β1) and collagens. The results showed that MPCs incorporated with dexamethasone and rhTGF-β1 increased proliferation and expression of glycosaminoglycan (GAG) after 14 days. Type II collagen enhanced the GAG synthesis, but did not increase alkaline phosphatase (ALP) activity. When adding dexamethasone and rhTGF-β1 MPCs increased mRNA expression of Sox9. Incorporation with type II collagen, dexamethasone and rhTGF-β1, MPCs induced mRNA expression of aggrecan and enhanced levels of type II collagen, and Sox9 mRNA. In contrast, incorporation with type I collagen, dexamethasone and rhTGF-β1 MPCs reduced levels of aggrecan, and Sox9 mRNA, and showed no type II collagen mRNA. Altogether, these results indicate that type I and II collagen, in addition to the cytokine effect, may play a functional role in regulating of chondrogenic differentiation by MPCs.
KW - Chondrogenic differentiation
KW - Collagen
KW - Dexamethasone
KW - Extracellular matrix
KW - Mesenchymal progenitor cells
KW - TGF-beta;1
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U2 - 10.1016/j.orthres.2004.09.002
DO - 10.1016/j.orthres.2004.09.002
M3 - Article
C2 - 15734261
AN - SCOPUS:14244252412
SN - 0736-0266
VL - 23
SP - 446
EP - 453
JO - Journal of Orthopaedic Research
JF - Journal of Orthopaedic Research
IS - 2
ER -