Abstract

In this study, a middle cerebral artery (MCA) occlusion mouse model is employed to study cerebral ischemia-reperfusion. A reproducible and reliable mouse model is useful for investigating the pathophysiology of cerebral ischemia-reperfusion and determining potential therapeutic strategies for patients with stroke. Variations in the anatomy of the circle of Willis of C57BL/6 mice affects their infarct volume after cerebralischemia- induced injury. Studies have indicated that distal MCA occlusion (MCAO) can overcome this problem and result in a stable infarct size. In this study, we establish a two-vessel occlusion mouse model of cerebral ischemia-reperfusion through the interruption of the blood flow to the right MCA. We distally ligate the right MCA and right common carotid artery (CCA) and restore blood flow after a certain period of ischemia. This ischemia-reperfusion injury induces an infarct of stable size and a behavioral deficit. Peripheral immune cells infiltrate the ischemic brain within the 24 h infiltration period. Additionally, the neuronal loss in the cortical area is less for a longer reperfusion duration. Therefore, this two-vessel occlusion model is suitable for investigating the immune response and neuronal recovery during the reperfusion period after cerebral ischemia.

Original languageEnglish
Article numbere59078
JournalJournal of Visualized Experiments
Volume2019
Issue number145
DOIs
Publication statusPublished - Mar 1 2019

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Brain Ischemia
Reperfusion
Middle Cerebral Artery Infarction
Middle Cerebral Artery
Blood
Circle of Willis
Infiltration
Common Carotid Artery
Brain
Reperfusion Injury
Inbred C57BL Mouse
Anatomy
Recovery
Ischemia
Stroke
Wounds and Injuries
Therapeutics

Keywords

  • 2,3,5-triphenyltetrazolium chloride assay
  • Cerebral ischemia-reperfusion middle cerebral artery occlusion
  • Imagej
  • Infarct volume
  • Issue 145
  • Medicine
  • Openfield assay

ASJC Scopus subject areas

  • Neuroscience(all)
  • Chemical Engineering(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Cite this

Two-vessel occlusion mouse model of cerebral ischemia-reperfusion. / Chen, Cheng Yu; Chen, Ray Jade; Lee, Gilbert Aaron.

In: Journal of Visualized Experiments, Vol. 2019, No. 145, e59078, 01.03.2019.

Research output: Contribution to journalArticle

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