Abstract

Background: Osteoporosis, a disease of the skeleton, results in an increased risk of fracture. Its characters are the loss of bone mass and degeneration of bone microstructure. This study was aimed to demonstrate an essential function of Twist-related protein 1 (TWIST1)-on osteoporosis. Methods: The expression of TWIST1 was overexpressed or silenced by specific transfection in MC3T3 cells and then were confirmed by real-time polymerase chain reaction (RT-PCR) and Western blot. The cell viability and cell migration were determined by 3-(4, 5-dimethyl-2-thiazolyl)-2, 5-diphenyl-2-H-tetrazolium bromide (MTT) and Transwell cell migration assay, respectively. Furthermore, cell apoptosis was analyzed by flow cytometry, and the protein expression of phosphatidyl inositide 3-kinases (PI3K) or p16 pathways related proteins was measured. Results: Here, this study showed that MC3T3 cells viability and migration were significantly increased by TWIST1 overexpression compared to the control group (P < 0.05), but the cell apoptosis was statistically decreased (P < 0.05). However, while TWIST1 silencing, the cells viability and migration were significantly appeared to be decreased, accompanied by apoptosis increasing (P < 0.05). Furthermore, the protein expression of PI3K and protein kinase B (p-Akt) were significantly increased (P < 0.05), but p16 and phosphorylate retinoblastoma protein (p-RB) were significantly decreased (P < 0.05). Conclusions: These results highlight the importance of TWIST1 to the target of treating osteoporosis. TWIST1 could promote cell viability and migration, but inhibit apoptosis in MC3T3 cells via regulating PI3K and p16 pathways.

Original languageEnglish
Pages (from-to)11269-11275
Number of pages7
JournalInternational Journal of Clinical and Experimental Pathology
Volume9
Issue number11
Publication statusPublished - 2016

Fingerprint

Twist-Related Protein 1
Cell Movement
Cell Survival
Phosphotransferases
Apoptosis
Osteoporosis
Cell Migration Assays
Bone and Bones
Retinoblastoma Protein
Proto-Oncogene Proteins c-akt
Proteins
Bromides
Skeleton
Transfection
Real-Time Polymerase Chain Reaction
Flow Cytometry
Western Blotting
Control Groups

Keywords

  • Apoptosis
  • Cell viability and migration
  • Osteoporosis
  • P16
  • Phosphatidyl inositide 3-kinases
  • Twist-related protein1

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Histology

Cite this

@article{aee53e820c8042378570aae95c60fb91,
title = "TWIST1 promotes cell viability and migration, but inhibits apoptosis in MC3T3 cells via regulating PI3K and p16 pathways",
abstract = "Background: Osteoporosis, a disease of the skeleton, results in an increased risk of fracture. Its characters are the loss of bone mass and degeneration of bone microstructure. This study was aimed to demonstrate an essential function of Twist-related protein 1 (TWIST1)-on osteoporosis. Methods: The expression of TWIST1 was overexpressed or silenced by specific transfection in MC3T3 cells and then were confirmed by real-time polymerase chain reaction (RT-PCR) and Western blot. The cell viability and cell migration were determined by 3-(4, 5-dimethyl-2-thiazolyl)-2, 5-diphenyl-2-H-tetrazolium bromide (MTT) and Transwell cell migration assay, respectively. Furthermore, cell apoptosis was analyzed by flow cytometry, and the protein expression of phosphatidyl inositide 3-kinases (PI3K) or p16 pathways related proteins was measured. Results: Here, this study showed that MC3T3 cells viability and migration were significantly increased by TWIST1 overexpression compared to the control group (P < 0.05), but the cell apoptosis was statistically decreased (P < 0.05). However, while TWIST1 silencing, the cells viability and migration were significantly appeared to be decreased, accompanied by apoptosis increasing (P < 0.05). Furthermore, the protein expression of PI3K and protein kinase B (p-Akt) were significantly increased (P < 0.05), but p16 and phosphorylate retinoblastoma protein (p-RB) were significantly decreased (P < 0.05). Conclusions: These results highlight the importance of TWIST1 to the target of treating osteoporosis. TWIST1 could promote cell viability and migration, but inhibit apoptosis in MC3T3 cells via regulating PI3K and p16 pathways.",
keywords = "Apoptosis, Cell viability and migration, Osteoporosis, P16, Phosphatidyl inositide 3-kinases, Twist-related protein1",
author = "Chen, {Chia Hsien} and Wu, {Jia Lin} and Lu, {Hsien Tsung} and Tsuang, {Yang Hwei} and Kuo, {Yi Jie}",
year = "2016",
language = "English",
volume = "9",
pages = "11269--11275",
journal = "International Journal of Clinical and Experimental Pathology",
issn = "1936-2625",
publisher = "E-CENTURY PUBLISHING CORP",
number = "11",

}

TY - JOUR

T1 - TWIST1 promotes cell viability and migration, but inhibits apoptosis in MC3T3 cells via regulating PI3K and p16 pathways

AU - Chen, Chia Hsien

AU - Wu, Jia Lin

AU - Lu, Hsien Tsung

AU - Tsuang, Yang Hwei

AU - Kuo, Yi Jie

PY - 2016

Y1 - 2016

N2 - Background: Osteoporosis, a disease of the skeleton, results in an increased risk of fracture. Its characters are the loss of bone mass and degeneration of bone microstructure. This study was aimed to demonstrate an essential function of Twist-related protein 1 (TWIST1)-on osteoporosis. Methods: The expression of TWIST1 was overexpressed or silenced by specific transfection in MC3T3 cells and then were confirmed by real-time polymerase chain reaction (RT-PCR) and Western blot. The cell viability and cell migration were determined by 3-(4, 5-dimethyl-2-thiazolyl)-2, 5-diphenyl-2-H-tetrazolium bromide (MTT) and Transwell cell migration assay, respectively. Furthermore, cell apoptosis was analyzed by flow cytometry, and the protein expression of phosphatidyl inositide 3-kinases (PI3K) or p16 pathways related proteins was measured. Results: Here, this study showed that MC3T3 cells viability and migration were significantly increased by TWIST1 overexpression compared to the control group (P < 0.05), but the cell apoptosis was statistically decreased (P < 0.05). However, while TWIST1 silencing, the cells viability and migration were significantly appeared to be decreased, accompanied by apoptosis increasing (P < 0.05). Furthermore, the protein expression of PI3K and protein kinase B (p-Akt) were significantly increased (P < 0.05), but p16 and phosphorylate retinoblastoma protein (p-RB) were significantly decreased (P < 0.05). Conclusions: These results highlight the importance of TWIST1 to the target of treating osteoporosis. TWIST1 could promote cell viability and migration, but inhibit apoptosis in MC3T3 cells via regulating PI3K and p16 pathways.

AB - Background: Osteoporosis, a disease of the skeleton, results in an increased risk of fracture. Its characters are the loss of bone mass and degeneration of bone microstructure. This study was aimed to demonstrate an essential function of Twist-related protein 1 (TWIST1)-on osteoporosis. Methods: The expression of TWIST1 was overexpressed or silenced by specific transfection in MC3T3 cells and then were confirmed by real-time polymerase chain reaction (RT-PCR) and Western blot. The cell viability and cell migration were determined by 3-(4, 5-dimethyl-2-thiazolyl)-2, 5-diphenyl-2-H-tetrazolium bromide (MTT) and Transwell cell migration assay, respectively. Furthermore, cell apoptosis was analyzed by flow cytometry, and the protein expression of phosphatidyl inositide 3-kinases (PI3K) or p16 pathways related proteins was measured. Results: Here, this study showed that MC3T3 cells viability and migration were significantly increased by TWIST1 overexpression compared to the control group (P < 0.05), but the cell apoptosis was statistically decreased (P < 0.05). However, while TWIST1 silencing, the cells viability and migration were significantly appeared to be decreased, accompanied by apoptosis increasing (P < 0.05). Furthermore, the protein expression of PI3K and protein kinase B (p-Akt) were significantly increased (P < 0.05), but p16 and phosphorylate retinoblastoma protein (p-RB) were significantly decreased (P < 0.05). Conclusions: These results highlight the importance of TWIST1 to the target of treating osteoporosis. TWIST1 could promote cell viability and migration, but inhibit apoptosis in MC3T3 cells via regulating PI3K and p16 pathways.

KW - Apoptosis

KW - Cell viability and migration

KW - Osteoporosis

KW - P16

KW - Phosphatidyl inositide 3-kinases

KW - Twist-related protein1

UR - http://www.scopus.com/inward/record.url?scp=85006168546&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85006168546&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:85006168546

VL - 9

SP - 11269

EP - 11275

JO - International Journal of Clinical and Experimental Pathology

JF - International Journal of Clinical and Experimental Pathology

SN - 1936-2625

IS - 11

ER -