Tumorous imaginal disc 1 (TID1) inhibits isoproterenol-induced cardiac hypertrophy and apoptosis by regulating c-terminus of hsc70-interacting protein (CHIP) mediated degradation of Gαs

Chih Chung Feng, Po Hsiang Liao, Hsiang I. Tsai, Shiu Min Cheng, Liang Yo Yang, Vijaya PadmaViswanadha, Lung Fa Pan, Ray Jade Chen, Jeng Fan Lo, Chih Yang Huang

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Dilated cardiomyopathy (DCM) is the most common form of non-ischemic cardiomyopathy. It is characterized by ventricular chamber dilation, and myocyte hypertrophy. Human tumorous imaginal disc 1 (Tid1), a chaperone protein and response to regulate number of signaling molecules in the mitochondria or cytosol. Tid1 also plays a major role in preventing DCM; however, the role of Tid1 in isoproterenol (ISO)-induced cardiac apoptosis and hypertrophy remains unclear. H9c2 cells were pretreated Tid1 before ISO-induced hypertrophy and apoptosis and then evaluated by IHC, TUNEL assay, IFC, Co-IP, and Western blot. From the IHC experiment, we found that Tid1 proteins were increased in tissues from different stages of human myocardial infarction. Using H9c2 cardiomyoblast cells we found that Tid1 was decreased by ISO treatment. However, over-expression of Tid1S suppressed NFATc3, BNP and calcineurin protein expression and inhibited NFATc3 nuclear translocation in ISO induced cardiomyoblast cells. On the other hand, Tid1S over-expression activated survival proteins p-AKTser473 and decreased caspase-3 and cytochrome c expression. We also found that overexpression of Tid1 enhanced CHIP expression, and induced CHIP to ubiquitinate Gαs, resulting in increased Gαs degradation. Our study showed that Gαs is a novel substrate of CHIP, and we also found that the Tid1-CHIP complex plays an essential role in inhibiting ISO induced cardiomyoblast hypertrophy and apoptosis.

Original languageEnglish
Pages (from-to)1537-1546
Number of pages10
JournalInternational Journal of Medical Sciences
Volume15
Issue number13
DOIs
Publication statusPublished - Jan 1 2018

    Fingerprint

Keywords

  • CHIP
  • Gαs
  • H9c2
  • hypertrophy
  • isoproterenol
  • Tid1

ASJC Scopus subject areas

  • Medicine(all)

Cite this