Tumor suppressor SCUBE2 inhibits breast-cancer cell migration and invasion through the reversal of epithelial-mesenchymal transition

Yuh Charn Lin, Yi Ching Lee, Ling Hui Li, Chien Jui Cheng, Ruey Bing Yang

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Signal peptide-CUB-EGF domain-containing protein 2 (SCUBE2) belongs to a secreted and membrane-associated multi-domainSCUBE protein family. We previously demonstrated that SCUBE2 is a novel breast-tumor suppressor and could be a useful prognosticmarker. However, the role of SCUBE2 in breast-cancer cellmigration and invasion and how it is regulated during theepithelial-mesenchymal transition (EMT) remain undefined. In thisstudy, we showed that ectopic SCUBE2 overexpression couldenhance the formation of E-cadherin-containing adherens junctionsby b-catenin-SOX-mediated induction of forkhead box A1 (apositive regulator of E-cadherin) and upregulation of E-cadherin, which in turn led to epithelial transition and inhibited migration and invasion of aggressive MDA-MB-231 breast-carcinoma cells. SCUBE2 expression was repressed together with that of E-cadherinin TGF-b-induced EMT; direct expression of SCUBE2 alone was sufficient to inhibit the TGF-b-induced EMT. Furthermore, quantitative DNA methylation, methylation-specific PCR, and chromatin immunoprecipitation analyses revealed that SCUBE2 expression was inactivated by DNA hypermethylation at the CpG islands by recruiting and binding DNA methyltransferase 1 during TGF-b-induced EMT. Together, our results suggest that SCUBE2 plays a key role in suppressing breast-carcinoma-cell mobility and invasiveness by increasing the formation of the epithelial E-cadherin-containing adherens junctions to promote epithelial differentiation and drive the reversal of EMT.

Original languageEnglish
Pages (from-to)85-100
Number of pages16
JournalJournal of Cell Science
Volume127
Issue number1
DOIs
Publication statusPublished - Jan 1 2014

Fingerprint

Epithelial-Mesenchymal Transition
Cadherins
Cell Movement
Breast Neoplasms
Neoplasms
Adherens Junctions
Catenins
CpG Islands
Chromatin Immunoprecipitation
DNA
Methyltransferases
DNA Methylation
Protein Sorting Signals
Epidermal Growth Factor
Methylation
Up-Regulation
Polymerase Chain Reaction
Membranes
Proteins

Keywords

  • Breast cancer
  • DNA hypermethylation
  • DNA methyltransferase
  • Epigenetic regulation
  • Epithelial-mesenchymal transition
  • Tumor suppressor

ASJC Scopus subject areas

  • Cell Biology

Cite this

Tumor suppressor SCUBE2 inhibits breast-cancer cell migration and invasion through the reversal of epithelial-mesenchymal transition. / Lin, Yuh Charn; Lee, Yi Ching; Li, Ling Hui; Cheng, Chien Jui; Yang, Ruey Bing.

In: Journal of Cell Science, Vol. 127, No. 1, 01.01.2014, p. 85-100.

Research output: Contribution to journalArticle

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