Trilinolein preserves mitochondria ultrastructure in isolated rat heart subjected to global ischemia through antioxidant activity as measured by chemiluminescence

Paul Chan, Chiang Shan Niu, Juei Tang Cheng, Chiung Wen Tsao, Shen Kou Tsai, Chuang Ye Hong

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Oxygen-derived free radicals (OFR) have been proposed as the cause of myocardial damage through lipid peroxidation during ischemia and reperfusion. Antioxidants can effectively ameliorate the damage induced by lipid peroxidation. Trilinolein is a triacylglycerol recently purified from the well known traditional Chinese herb Panax pseudoginseng, which has been used in treating circulatory disorders among Chinese for hundreds of years; it has linoleate as the only fatty acid residue in all three esterified positions of glycerol. This chemical has recently been demonstrated to have antioxidant activity by enhanced chemiluminescence. The addition of phorbol myristic acetate (PMA) to medium containing leukocytes produces OFR; this phenomenon was measured by chemiluminescence. Addition of trilinolein to medium containing leukocytes preceding the addition of PMA suppressed the production of OFR. The control value of chemiluminescence of a medium containing leukocytes with addition of PMA was 9.23 ± 1.19 x 103 mV. The most effective concentration of trilinolein was 10-7 mol/l which decreased the signals to 4.59 ± 0.02 x 103 mV (p <0.001). The antioxidant effect had a concentration-response curve similar to α-tocopherol. After pretreatment for 15 min with trilinolein at a concentration of 10-7 mol/l in isolated perfused rat heart which had been subjected to 60 min of global ischemia, the integrity of the rat heart mitochondria was preserved as examined under the electron microscope. No swelling of mitochondria occurred and there was good alignment of cristae and absence of amorphous density. Previous experiments have shown that trilinolein can also improve erythrocyte deformability in vitro. Infarct size reduction of about 50% was also demonstrated in in vivo rat heart subjected to 4 h coronary occlusion. The mechanism of myocardial protection, in addition to the antioxidant effect, is suggested as maintaining the membrane fluidity of cardiomyocytes.

Original languageEnglish
Pages (from-to)216-225
Number of pages10
JournalPharmacology
Volume52
Issue number4
Publication statusPublished - Apr 1996
Externally publishedYes

Fingerprint

Luminescence
Mitochondria
Ischemia
Antioxidants
Free Radicals
Acetates
Leukocytes
Oxygen
Lipid Peroxidation
Erythrocyte Deformability
Heart Mitochondria
Panax
Membrane Fluidity
Tocopherols
Coronary Occlusion
Linoleic Acid
Cardiac Myocytes
Glycerol
Reperfusion
Triglycerides

Keywords

  • Chemiluminescence
  • Free radicals
  • Ischemia
  • Mitochondria
  • Trilinolein

ASJC Scopus subject areas

  • Pharmacology

Cite this

Trilinolein preserves mitochondria ultrastructure in isolated rat heart subjected to global ischemia through antioxidant activity as measured by chemiluminescence. / Chan, Paul; Niu, Chiang Shan; Cheng, Juei Tang; Tsao, Chiung Wen; Tsai, Shen Kou; Hong, Chuang Ye.

In: Pharmacology, Vol. 52, No. 4, 04.1996, p. 216-225.

Research output: Contribution to journalArticle

Chan, Paul ; Niu, Chiang Shan ; Cheng, Juei Tang ; Tsao, Chiung Wen ; Tsai, Shen Kou ; Hong, Chuang Ye. / Trilinolein preserves mitochondria ultrastructure in isolated rat heart subjected to global ischemia through antioxidant activity as measured by chemiluminescence. In: Pharmacology. 1996 ; Vol. 52, No. 4. pp. 216-225.
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T1 - Trilinolein preserves mitochondria ultrastructure in isolated rat heart subjected to global ischemia through antioxidant activity as measured by chemiluminescence

AU - Chan, Paul

AU - Niu, Chiang Shan

AU - Cheng, Juei Tang

AU - Tsao, Chiung Wen

AU - Tsai, Shen Kou

AU - Hong, Chuang Ye

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N2 - Oxygen-derived free radicals (OFR) have been proposed as the cause of myocardial damage through lipid peroxidation during ischemia and reperfusion. Antioxidants can effectively ameliorate the damage induced by lipid peroxidation. Trilinolein is a triacylglycerol recently purified from the well known traditional Chinese herb Panax pseudoginseng, which has been used in treating circulatory disorders among Chinese for hundreds of years; it has linoleate as the only fatty acid residue in all three esterified positions of glycerol. This chemical has recently been demonstrated to have antioxidant activity by enhanced chemiluminescence. The addition of phorbol myristic acetate (PMA) to medium containing leukocytes produces OFR; this phenomenon was measured by chemiluminescence. Addition of trilinolein to medium containing leukocytes preceding the addition of PMA suppressed the production of OFR. The control value of chemiluminescence of a medium containing leukocytes with addition of PMA was 9.23 ± 1.19 x 103 mV. The most effective concentration of trilinolein was 10-7 mol/l which decreased the signals to 4.59 ± 0.02 x 103 mV (p <0.001). The antioxidant effect had a concentration-response curve similar to α-tocopherol. After pretreatment for 15 min with trilinolein at a concentration of 10-7 mol/l in isolated perfused rat heart which had been subjected to 60 min of global ischemia, the integrity of the rat heart mitochondria was preserved as examined under the electron microscope. No swelling of mitochondria occurred and there was good alignment of cristae and absence of amorphous density. Previous experiments have shown that trilinolein can also improve erythrocyte deformability in vitro. Infarct size reduction of about 50% was also demonstrated in in vivo rat heart subjected to 4 h coronary occlusion. The mechanism of myocardial protection, in addition to the antioxidant effect, is suggested as maintaining the membrane fluidity of cardiomyocytes.

AB - Oxygen-derived free radicals (OFR) have been proposed as the cause of myocardial damage through lipid peroxidation during ischemia and reperfusion. Antioxidants can effectively ameliorate the damage induced by lipid peroxidation. Trilinolein is a triacylglycerol recently purified from the well known traditional Chinese herb Panax pseudoginseng, which has been used in treating circulatory disorders among Chinese for hundreds of years; it has linoleate as the only fatty acid residue in all three esterified positions of glycerol. This chemical has recently been demonstrated to have antioxidant activity by enhanced chemiluminescence. The addition of phorbol myristic acetate (PMA) to medium containing leukocytes produces OFR; this phenomenon was measured by chemiluminescence. Addition of trilinolein to medium containing leukocytes preceding the addition of PMA suppressed the production of OFR. The control value of chemiluminescence of a medium containing leukocytes with addition of PMA was 9.23 ± 1.19 x 103 mV. The most effective concentration of trilinolein was 10-7 mol/l which decreased the signals to 4.59 ± 0.02 x 103 mV (p <0.001). The antioxidant effect had a concentration-response curve similar to α-tocopherol. After pretreatment for 15 min with trilinolein at a concentration of 10-7 mol/l in isolated perfused rat heart which had been subjected to 60 min of global ischemia, the integrity of the rat heart mitochondria was preserved as examined under the electron microscope. No swelling of mitochondria occurred and there was good alignment of cristae and absence of amorphous density. Previous experiments have shown that trilinolein can also improve erythrocyte deformability in vitro. Infarct size reduction of about 50% was also demonstrated in in vivo rat heart subjected to 4 h coronary occlusion. The mechanism of myocardial protection, in addition to the antioxidant effect, is suggested as maintaining the membrane fluidity of cardiomyocytes.

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