Trilinolein inhibits proliferation of human non-small cell lung carcinoma A549 through the modulation of PI3K/Akt pathway

Pei Yu Chou, Guan Jhong Huang, Chun Hsu Pan, Yi Chung Chien, Ying Yi Chen, Chieh Hsi Wu, Ming Jyh Sheu, Hsu Chen Cheng

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Trilinolein has been identified as one of the active constituents isolated from Panax notoginseng used widely in traditional Chinese medicine. Protective actions of Panax notoginseng against cerebral ischemia, beneficial effects on the cardiovascular system, and hemostatic, antioxidant, hypolipidemic, hepatoprotective, renoprotective and estrogen-like activities have been illustrated. In the present study, the effects of trilinolein on the growth of non-small cell lung carcinoma A549 were investigated. It was found that the exposure of A549 cells to trilinolein resulted in the growth inhibition and the induction of apoptosis in a dose- and time- dependent manner. Trilinolein treatment induced the upregulation of pro-apoptotic Bax, downregulation of anti-apoptotic Bcl-2 expression, which was associated with the proteolytic activation of caspases and the concomitant degradation of poly(ADP-ribose) polymerase (PARP) protein. Intracellular reactive oxygen species seem to play a role in the trilinolein-induced apoptosis, since ROS were produced early in the trilinolein treatment. Moreover, the activity of PI3K/Akt was downregulated in trilinolein-treated cells. Our results demonstrated that the most important regulators of trilinolein-induced apoptosis are Bcl-2 family and caspase-3, which are associated with cytochrome c release and dephosphorylation on the Akt signaling pathway.

Original languageEnglish
Pages (from-to)803-815
Number of pages13
JournalAmerican Journal of Chinese Medicine
Volume39
Issue number4
DOIs
Publication statusPublished - 2011
Externally publishedYes

Keywords

  • Apoptosis
  • Chinese Herbs
  • Panax notoginseng
  • PI3K/Akt
  • Trilinolein

ASJC Scopus subject areas

  • Complementary and alternative medicine

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