Triflavin, an RGD-containing antiplatelet peptide, binds to GPIIIa of ADP-stimulated platelets

Joen Rong Sheu, Che Ming Teng, Tur Fu Huang

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

Triflavin, an Arg-Gly-Asp-containing snake venom peptide, inhibits platelet aggregation through the blockade of fibrinogen binding to the activated platelets. It binds to fibrinogen receptors associated with the glycoprotein IIb/IIIa complex with a Kd value of 7×10-8 M. In this report, a chemical cross-linking approach was used to further characterize the binding components of triflavin on platelet membrane. 125I-triflavin binding was performed with the aid of a chemical cross-linking reagent, DTSSP. Analysis of the cross-linked products by SDS-PAGE (7.5% gel) and subsequent autoradiogram revealed that 125I-triflavin was cross-linked specifically to a protein with an apparent molecular weight of 1.1×105, and this reaction was inhibited by GRDGS and excess of non-labeled triflavin. This 110 KDa component was identified to be GpIIIa, recognized by AP3, a mAb against GpIIIa, by immunoblotting technique. These results indicate that the triflavin-binding sites on platelets reside at a site in close proximity to GpIIIa.

Original languageEnglish
Pages (from-to)1236-1242
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume189
Issue number2
DOIs
Publication statusPublished - Dec 15 1992
Externally publishedYes

Fingerprint

Integrin beta3
Platelets
Adenosine Diphosphate
Blood Platelets
Peptides
Fibrinogen Receptors
Cross-Linking Reagents
Platelet Glycoprotein GPIIb-IIIa Complex
Snake Venoms
Platelet Aggregation
Immunoblotting
Fibrinogen
triflavin
Polyacrylamide Gel Electrophoresis
Agglomeration
Molecular Weight
Gels
Molecular weight
Binding Sites
Membranes

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Triflavin, an RGD-containing antiplatelet peptide, binds to GPIIIa of ADP-stimulated platelets. / Sheu, Joen Rong; Teng, Che Ming; Huang, Tur Fu.

In: Biochemical and Biophysical Research Communications, Vol. 189, No. 2, 15.12.1992, p. 1236-1242.

Research output: Contribution to journalArticle

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