Triflavin, an Arg-Gly-Asp-containing peptide, prevents platelet plug formation in in vivo experiments

Joen Rong Sheu, Mao Hsiung Yen, Hui Chin Peng, Mei Chi Chang, Tur Fu Huang

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Triflavin, an Arg-Gly-Asp-containing peptide from Trimeresurus flauoviridis snake venom (M1, of 7500 Da) inhibits platelet aggregation through the blockade of fibrinogen binding to activated platelets. The present study demonstrated that the intravenous injection of triflavin (0.1 and 0.25 mg/kg) significantly prolonged the bleeding time about 1.8- to 2.4-fold as compared with control (normal saline) of severed mesenteric arteries in rats, whereas the injection of Gly-Arg-Gly-Asp-Ser (GRGDS) (2-8 mg/kg) failed to increase the bleeding time in this model. Continuous infusion of triflavin (0.08 mg/kg/min) significantly increased the bleeding time about 2.6-fold, and the bleeding time returned to normal within 20 min after the cessation of triflavin infusion. Triflavin (10-20 mg/kg) significantly prolonged the occlusion time of platelet plug formation induced by irradiation of mesenteric venules of fluorescein sodium-pretreated mice. In contrast, trigramin (10-20 mg/kg) and GRGDS (500 and 1000 mg/kg) showed no significant effect. These results suggest that triflavin has an effective antiplatelet effect in vivo and this peptide may be a useful therapeutic agent for arterial thrombosis.

Original languageEnglish
Pages (from-to)231-238
Number of pages8
JournalEuropean Journal of Pharmacology
Volume294
Issue number1
DOIs
Publication statusPublished - Dec 27 1995

Keywords

  • Bleeding time
  • Fibrinogen receptor antagonist
  • Mesenteric artery
  • RGD (Arg-Gly-Asp)-containing peptide
  • Thrombosis
  • Triflavin

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Pharmacology

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