Abstract
Vascular smooth muscle cell (VSMC) adhesion and migration play important roles in atherosclerosis and intimal hyperplasia. In our previous study, we found that triflavin, a nonspecific Arg-Gly-Asp (RGD)-containing peptide (also named αIIβ3 disintegrin), may have dual beneficial effects in preventing neointimal formation by acting on both platelets and VSMCs, which has created new incentives for the development of drugs with this combined action. In the present study, triflavin (10, 20, and 50μg/mL) concentration- dependently inhibited VSMC adhesion to immobilized fibronectin (50μg/mL). In the flow cytometric study, we found that FITCtriflavin (5μg/mL) bound directly to VSMC membranes. In a confocal microscopic study, fibronectin (50μg/mL) markedly stimulated protein kinase C (PKC)-α translocation from the cytosol to the membranes, which was abolished in the presence of triflavin (10μg/mL). In conclusion, the most important findings of this study suggest that triflavin, an αIIβ3 disintegrin, inhibited immobilized fibronectin-induced cell adhesion and PKC-α translocation in VSMCs.
| Original language | English |
|---|---|
| Pages (from-to) | 948-954 |
| Number of pages | 7 |
| Journal | Pharmaceutical Biology |
| Volume | 47 |
| Issue number | 10 |
| DOIs | |
| Publication status | Published - Oct 2009 |
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Keywords
- Fibronectin
- PKC-α
- Triflavin
- Vascular smooth muscle cell
ASJC Scopus subject areas
- Molecular Medicine
- Complementary and alternative medicine
- Drug Discovery
- Pharmaceutical Science
- Pharmacology
Cite this
Triflavin, an αiIβ3 disintegrin, inhibits cell adhesion and protein kinase C-α translocation in vascular smooth muscle cells. / Wu, Chih J.; Hsieh, Cheng Ying; Hsiao, George; Chou, Duen Suey; Sheu, Joen Rong.
In: Pharmaceutical Biology, Vol. 47, No. 10, 10.2009, p. 948-954.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Triflavin, an αiIβ3 disintegrin, inhibits cell adhesion and protein kinase C-α translocation in vascular smooth muscle cells
AU - Wu, Chih J.
AU - Hsieh, Cheng Ying
AU - Hsiao, George
AU - Chou, Duen Suey
AU - Sheu, Joen Rong
PY - 2009/10
Y1 - 2009/10
N2 - Vascular smooth muscle cell (VSMC) adhesion and migration play important roles in atherosclerosis and intimal hyperplasia. In our previous study, we found that triflavin, a nonspecific Arg-Gly-Asp (RGD)-containing peptide (also named αIIβ3 disintegrin), may have dual beneficial effects in preventing neointimal formation by acting on both platelets and VSMCs, which has created new incentives for the development of drugs with this combined action. In the present study, triflavin (10, 20, and 50μg/mL) concentration- dependently inhibited VSMC adhesion to immobilized fibronectin (50μg/mL). In the flow cytometric study, we found that FITCtriflavin (5μg/mL) bound directly to VSMC membranes. In a confocal microscopic study, fibronectin (50μg/mL) markedly stimulated protein kinase C (PKC)-α translocation from the cytosol to the membranes, which was abolished in the presence of triflavin (10μg/mL). In conclusion, the most important findings of this study suggest that triflavin, an αIIβ3 disintegrin, inhibited immobilized fibronectin-induced cell adhesion and PKC-α translocation in VSMCs.
AB - Vascular smooth muscle cell (VSMC) adhesion and migration play important roles in atherosclerosis and intimal hyperplasia. In our previous study, we found that triflavin, a nonspecific Arg-Gly-Asp (RGD)-containing peptide (also named αIIβ3 disintegrin), may have dual beneficial effects in preventing neointimal formation by acting on both platelets and VSMCs, which has created new incentives for the development of drugs with this combined action. In the present study, triflavin (10, 20, and 50μg/mL) concentration- dependently inhibited VSMC adhesion to immobilized fibronectin (50μg/mL). In the flow cytometric study, we found that FITCtriflavin (5μg/mL) bound directly to VSMC membranes. In a confocal microscopic study, fibronectin (50μg/mL) markedly stimulated protein kinase C (PKC)-α translocation from the cytosol to the membranes, which was abolished in the presence of triflavin (10μg/mL). In conclusion, the most important findings of this study suggest that triflavin, an αIIβ3 disintegrin, inhibited immobilized fibronectin-induced cell adhesion and PKC-α translocation in VSMCs.
KW - Fibronectin
KW - PKC-α
KW - Triflavin
KW - Vascular smooth muscle cell
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U2 - 10.1080/13880200902956683
DO - 10.1080/13880200902956683
M3 - Article
VL - 47
SP - 948
EP - 954
JO - Pharmaceutical Biology
JF - Pharmaceutical Biology
SN - 1388-0209
IS - 10
ER -