Trichodermin induces c-Jun N-terminal kinase-dependent apoptosis caused by mitotic arrest and DNA damage in human p53-mutated pancreatic cancer cells and xenografts

Ming Hsien Chien, Tzong Huei Lee, Wei Jiunn Lee, Yen Hsiu Yeh, Tsai Kun Li, Po Chuan Wang, Jih Jung Chen, Jyh Ming Chow, Yung Wei Lin, Michael Hsiao, Shih Wei Wang, Kuo Tai Hua

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Pancreatic cancer is an aggressive malignancy, which generally responds poorly to chemotherapy. In this study, trichodermin, an endophytic fungal metabolite from Nalanthamala psidii, was identified as a potent and selective antitumor agent in human pancreatic cancer. Trichodermin exhibited antiproliferative effects against pancreatic cancer cells, especially p53-mutated cells (MIA PaCa-2 and BxPC-3) rather than normal pancreatic epithelial cells. We found that trichodermin induced caspase-dependent and mitochondrial intrinsic apoptosis. Trichodermin also increased apoptosis through mitotic arrest by activating Cdc2/cyclin B1 complex activity. Moreover, trichodermin promoted the activation of c-Jun N-terminal kinase (JNK), and inhibition of JNK by its inhibitor, shRNA, or siRNA significantly reversed trichodermin-mediated caspase-dependent apoptosis. Trichodermin triggered DNA damage stress to activate p53 function for executing apoptosis in p53-mutated cells. Importantly, we demonstrated that trichodermin with efficacy similar to gemcitabine, profoundly suppressed tumor growth through inducing intratumoral DNA damage and JNK activation in orthotopic pancreatic cancer model. Based on these findings, trichodermin is a potential therapeutic agent worthy of further development into a clinical trial candidate for treating cancer, especially the mutant p53 pancreatic cancer.

Original languageEnglish
Pages (from-to)249-261
Number of pages13
JournalCancer Letters
Volume388
DOIs
Publication statusPublished - Mar 1 2017

Fingerprint

Trichodermin
JNK Mitogen-Activated Protein Kinases
Pancreatic Neoplasms
Heterografts
DNA Damage
Apoptosis
gemcitabine
Caspases
Small Interfering RNA
Cyclin B1
Neoplasms
Antineoplastic Agents

Keywords

  • Apoptosis
  • c-Jun N-terminal kinase
  • DNA damage
  • Mitotic arrest
  • Pancreatic cancer
  • Trichodermin

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Trichodermin induces c-Jun N-terminal kinase-dependent apoptosis caused by mitotic arrest and DNA damage in human p53-mutated pancreatic cancer cells and xenografts. / Chien, Ming Hsien; Lee, Tzong Huei; Lee, Wei Jiunn; Yeh, Yen Hsiu; Li, Tsai Kun; Wang, Po Chuan; Chen, Jih Jung; Chow, Jyh Ming; Lin, Yung Wei; Hsiao, Michael; Wang, Shih Wei; Hua, Kuo Tai.

In: Cancer Letters, Vol. 388, 01.03.2017, p. 249-261.

Research output: Contribution to journalArticle

Chien, Ming Hsien ; Lee, Tzong Huei ; Lee, Wei Jiunn ; Yeh, Yen Hsiu ; Li, Tsai Kun ; Wang, Po Chuan ; Chen, Jih Jung ; Chow, Jyh Ming ; Lin, Yung Wei ; Hsiao, Michael ; Wang, Shih Wei ; Hua, Kuo Tai. / Trichodermin induces c-Jun N-terminal kinase-dependent apoptosis caused by mitotic arrest and DNA damage in human p53-mutated pancreatic cancer cells and xenografts. In: Cancer Letters. 2017 ; Vol. 388. pp. 249-261.
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AU - Lee, Wei Jiunn

AU - Yeh, Yen Hsiu

AU - Li, Tsai Kun

AU - Wang, Po Chuan

AU - Chen, Jih Jung

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AU - Hua, Kuo Tai

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AB - Pancreatic cancer is an aggressive malignancy, which generally responds poorly to chemotherapy. In this study, trichodermin, an endophytic fungal metabolite from Nalanthamala psidii, was identified as a potent and selective antitumor agent in human pancreatic cancer. Trichodermin exhibited antiproliferative effects against pancreatic cancer cells, especially p53-mutated cells (MIA PaCa-2 and BxPC-3) rather than normal pancreatic epithelial cells. We found that trichodermin induced caspase-dependent and mitochondrial intrinsic apoptosis. Trichodermin also increased apoptosis through mitotic arrest by activating Cdc2/cyclin B1 complex activity. Moreover, trichodermin promoted the activation of c-Jun N-terminal kinase (JNK), and inhibition of JNK by its inhibitor, shRNA, or siRNA significantly reversed trichodermin-mediated caspase-dependent apoptosis. Trichodermin triggered DNA damage stress to activate p53 function for executing apoptosis in p53-mutated cells. Importantly, we demonstrated that trichodermin with efficacy similar to gemcitabine, profoundly suppressed tumor growth through inducing intratumoral DNA damage and JNK activation in orthotopic pancreatic cancer model. Based on these findings, trichodermin is a potential therapeutic agent worthy of further development into a clinical trial candidate for treating cancer, especially the mutant p53 pancreatic cancer.

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