Triage of atypical glandular cell by SOX1 and POU4F3 methylation

A Taiwanese Gynecologic Oncology Group (TGOG) study

Cheng Chang Chang, Yu Che Ou, Kung Liahng Wang, Ting Chang Chang, Ya Min Cheng, Chi Hau Chen, Tang Yuan Chu, Shih Tien Hsu, Wen Shiung Liou, Yin Yi Chang, Hua Hsi Wu, Tze Ho Chen, Hung Cheng Lai

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Introduction: Invasive procedures including loop electrosurgical excision, cervical conization, and endometrial sampling are often recommended when atypical glandular cells (AGC) are detected on Pap smear with unsatisfactory colposcopy. These invasive procedures may result in patient anxiety, increased medical expense, and increasing the risk of preterm delivery in subsequent pregnancies. This study was performed to assess methylation biomarkers in the triage of AGC on Pap smear for invasive procedures. Methods: We conducted a multicenter study in 13 medical centers in Taiwan from May 2012 to May 2014. A total of 55 samples diagnosed "AGC not otherwise specified" (AGC-NOS) were included. All patients with AGC underwent colposcopy, cervical biopsy, endometrial sampling, and conization if indicated. Multiplex quantitative methylation-specific polymerase chain reaction (QMSPCR) was performed. Sensitivity, specificity, and accuracy were calculated for detecting CIN3+ and endometrial complex hyperplasia. Results: In 55 patients with AGC, the sensitivity for methylated (m) SOX1m, PAX1m, ZNF582m, PTPRRm, AJAP1m, HS3ST2m, and POU4F3m for detecting CIN3+ and endometrial complex hyperplasia lesions was 100, 86, 71, 86, 86, 57, and 100%; specificity was 67, 79, 85, 50, 52, 96, and 52%, respectively. Testing for high risk-HPV had a sensitivity of 57% and specificity of 75% for CIN3+ and endometrial complex hyperplasia lesions. Conclusion: Methylated (m) SOX1mand POU4F3mcould be new methylation biomarkers for detection of CIN3+ and endometrial complex hyperplasia in AGC. Women with AGC and positive SOX1m/ POU4F3m, colposcopy, cervical conization or endometrial sampling should be considered.

Original languageEnglish
Article numbere0128705
JournalPLoS One
Volume10
Issue number6
DOIs
Publication statusPublished - Jun 9 2015

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Methylation
Oncology
Triage
methylation
Endometrial Hyperplasia
Biomarkers
Sampling
colposcopy
Conization
hyperplasia
Colposcopy
Biopsy
Polymerase chain reaction
Papanicolaou Test
cells
lesions (animal)
biomarkers
Sensitivity and Specificity
sampling
Testing

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Triage of atypical glandular cell by SOX1 and POU4F3 methylation : A Taiwanese Gynecologic Oncology Group (TGOG) study. / Chang, Cheng Chang; Ou, Yu Che; Wang, Kung Liahng; Chang, Ting Chang; Cheng, Ya Min; Chen, Chi Hau; Chu, Tang Yuan; Hsu, Shih Tien; Liou, Wen Shiung; Chang, Yin Yi; Wu, Hua Hsi; Chen, Tze Ho; Lai, Hung Cheng.

In: PLoS One, Vol. 10, No. 6, e0128705, 09.06.2015.

Research output: Contribution to journalArticle

Chang, CC, Ou, YC, Wang, KL, Chang, TC, Cheng, YM, Chen, CH, Chu, TY, Hsu, ST, Liou, WS, Chang, YY, Wu, HH, Chen, TH & Lai, HC 2015, 'Triage of atypical glandular cell by SOX1 and POU4F3 methylation: A Taiwanese Gynecologic Oncology Group (TGOG) study', PLoS One, vol. 10, no. 6, e0128705. https://doi.org/10.1371/journal.pone.0128705
Chang, Cheng Chang ; Ou, Yu Che ; Wang, Kung Liahng ; Chang, Ting Chang ; Cheng, Ya Min ; Chen, Chi Hau ; Chu, Tang Yuan ; Hsu, Shih Tien ; Liou, Wen Shiung ; Chang, Yin Yi ; Wu, Hua Hsi ; Chen, Tze Ho ; Lai, Hung Cheng. / Triage of atypical glandular cell by SOX1 and POU4F3 methylation : A Taiwanese Gynecologic Oncology Group (TGOG) study. In: PLoS One. 2015 ; Vol. 10, No. 6.
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abstract = "Introduction: Invasive procedures including loop electrosurgical excision, cervical conization, and endometrial sampling are often recommended when atypical glandular cells (AGC) are detected on Pap smear with unsatisfactory colposcopy. These invasive procedures may result in patient anxiety, increased medical expense, and increasing the risk of preterm delivery in subsequent pregnancies. This study was performed to assess methylation biomarkers in the triage of AGC on Pap smear for invasive procedures. Methods: We conducted a multicenter study in 13 medical centers in Taiwan from May 2012 to May 2014. A total of 55 samples diagnosed {"}AGC not otherwise specified{"} (AGC-NOS) were included. All patients with AGC underwent colposcopy, cervical biopsy, endometrial sampling, and conization if indicated. Multiplex quantitative methylation-specific polymerase chain reaction (QMSPCR) was performed. Sensitivity, specificity, and accuracy were calculated for detecting CIN3+ and endometrial complex hyperplasia. Results: In 55 patients with AGC, the sensitivity for methylated (m) SOX1m, PAX1m, ZNF582m, PTPRRm, AJAP1m, HS3ST2m, and POU4F3m for detecting CIN3+ and endometrial complex hyperplasia lesions was 100, 86, 71, 86, 86, 57, and 100{\%}; specificity was 67, 79, 85, 50, 52, 96, and 52{\%}, respectively. Testing for high risk-HPV had a sensitivity of 57{\%} and specificity of 75{\%} for CIN3+ and endometrial complex hyperplasia lesions. Conclusion: Methylated (m) SOX1mand POU4F3mcould be new methylation biomarkers for detection of CIN3+ and endometrial complex hyperplasia in AGC. Women with AGC and positive SOX1m/ POU4F3m, colposcopy, cervical conization or endometrial sampling should be considered.",
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T1 - Triage of atypical glandular cell by SOX1 and POU4F3 methylation

T2 - A Taiwanese Gynecologic Oncology Group (TGOG) study

AU - Chang, Cheng Chang

AU - Ou, Yu Che

AU - Wang, Kung Liahng

AU - Chang, Ting Chang

AU - Cheng, Ya Min

AU - Chen, Chi Hau

AU - Chu, Tang Yuan

AU - Hsu, Shih Tien

AU - Liou, Wen Shiung

AU - Chang, Yin Yi

AU - Wu, Hua Hsi

AU - Chen, Tze Ho

AU - Lai, Hung Cheng

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N2 - Introduction: Invasive procedures including loop electrosurgical excision, cervical conization, and endometrial sampling are often recommended when atypical glandular cells (AGC) are detected on Pap smear with unsatisfactory colposcopy. These invasive procedures may result in patient anxiety, increased medical expense, and increasing the risk of preterm delivery in subsequent pregnancies. This study was performed to assess methylation biomarkers in the triage of AGC on Pap smear for invasive procedures. Methods: We conducted a multicenter study in 13 medical centers in Taiwan from May 2012 to May 2014. A total of 55 samples diagnosed "AGC not otherwise specified" (AGC-NOS) were included. All patients with AGC underwent colposcopy, cervical biopsy, endometrial sampling, and conization if indicated. Multiplex quantitative methylation-specific polymerase chain reaction (QMSPCR) was performed. Sensitivity, specificity, and accuracy were calculated for detecting CIN3+ and endometrial complex hyperplasia. Results: In 55 patients with AGC, the sensitivity for methylated (m) SOX1m, PAX1m, ZNF582m, PTPRRm, AJAP1m, HS3ST2m, and POU4F3m for detecting CIN3+ and endometrial complex hyperplasia lesions was 100, 86, 71, 86, 86, 57, and 100%; specificity was 67, 79, 85, 50, 52, 96, and 52%, respectively. Testing for high risk-HPV had a sensitivity of 57% and specificity of 75% for CIN3+ and endometrial complex hyperplasia lesions. Conclusion: Methylated (m) SOX1mand POU4F3mcould be new methylation biomarkers for detection of CIN3+ and endometrial complex hyperplasia in AGC. Women with AGC and positive SOX1m/ POU4F3m, colposcopy, cervical conization or endometrial sampling should be considered.

AB - Introduction: Invasive procedures including loop electrosurgical excision, cervical conization, and endometrial sampling are often recommended when atypical glandular cells (AGC) are detected on Pap smear with unsatisfactory colposcopy. These invasive procedures may result in patient anxiety, increased medical expense, and increasing the risk of preterm delivery in subsequent pregnancies. This study was performed to assess methylation biomarkers in the triage of AGC on Pap smear for invasive procedures. Methods: We conducted a multicenter study in 13 medical centers in Taiwan from May 2012 to May 2014. A total of 55 samples diagnosed "AGC not otherwise specified" (AGC-NOS) were included. All patients with AGC underwent colposcopy, cervical biopsy, endometrial sampling, and conization if indicated. Multiplex quantitative methylation-specific polymerase chain reaction (QMSPCR) was performed. Sensitivity, specificity, and accuracy were calculated for detecting CIN3+ and endometrial complex hyperplasia. Results: In 55 patients with AGC, the sensitivity for methylated (m) SOX1m, PAX1m, ZNF582m, PTPRRm, AJAP1m, HS3ST2m, and POU4F3m for detecting CIN3+ and endometrial complex hyperplasia lesions was 100, 86, 71, 86, 86, 57, and 100%; specificity was 67, 79, 85, 50, 52, 96, and 52%, respectively. Testing for high risk-HPV had a sensitivity of 57% and specificity of 75% for CIN3+ and endometrial complex hyperplasia lesions. Conclusion: Methylated (m) SOX1mand POU4F3mcould be new methylation biomarkers for detection of CIN3+ and endometrial complex hyperplasia in AGC. Women with AGC and positive SOX1m/ POU4F3m, colposcopy, cervical conization or endometrial sampling should be considered.

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