Trench-shaped binding sites promote multiple classes of interactions between collagen and the adherence receptors, α1β1 integrin and Staphylococcus aureus Cna MSCRAMM

Rebecca L. Rich, Champion C S Deivanayagam, Rick T. Owens, Michael Carson, Agneta Höök, Dwight Moore, Vivian W C Yang, Sthanam V L Narayana, Magnus Höök

Research output: Contribution to journalArticle

78 Citations (Scopus)

Abstract

Most mammalian cells and some pathogenic bacteria are capable of adhering to collagenous substrates in processes mediated by specific cell surface adherence molecules. Crystal structures of collagen-binding regions of the human integrin α2β1 and a Staphylococcus aureus adhesin reveal a 'trench' on the surface of both of these proteins. This trench can accommodate a collagen triple-helical structure and presumably represents the ligand-binding site (Emsley, J., King, S. L., Bergelson, J. M., and Liddington, R. C. (1997) J. Biol. Chem. 272, 28512-28517; Symersky, J., Patti, J. M., Carson, M., House-Pompeo, K., Teale, M., Moore, D., Jin, L., Schneider, A., DeLucas, L. J., Hook, M., and Narayana, S. V. L. (1997) Nat. Struct. Biol. 4, 833-838). We report here the crystal structure of the α subunit I domain from the α1β1 integrin. This collagen-binding protein also contains a trench on one face in which the collagen triple helix may be docked. Furthermore, we compare the collagen-binding mechanisms of the human α1 integrin I domain and the A domain from the S. aureus collagen adhesin, Cna. Although the S. aureus and human proteins have unrelated amino acid sequences, secondary structure composition, and cation requirements for effective ligand binding, both proteins bind at multiple sites within one collagen molecule, with the sites in collagen varying in their affinity for the adherence molecule. We propose that (i) these evolutionarily dissimilar adherence proteins recognize collagen via similar mechanisms, (ii) the multisite, multiclass protein/ligand interactions observed in these two systems result from a binding-site trench, and (iii) this unusual binding mechanism may be thematic for proteins binding extended, rigid ligands that contain repeating structural motifs.

Original languageEnglish
Pages (from-to)24906-24913
Number of pages8
JournalJournal of Biological Chemistry
Volume274
Issue number35
DOIs
Publication statusPublished - Aug 27 1999
Externally publishedYes

Fingerprint

Collagen Receptors
Integrins
Staphylococcus aureus
Collagen
Binding Sites
Ligands
Molecules
Carrier Proteins
Crystal structure
Proteins
Protein Binding
Cations
Amino Acid Sequence
Bacteria
Membrane Proteins
Cells

ASJC Scopus subject areas

  • Biochemistry

Cite this

Trench-shaped binding sites promote multiple classes of interactions between collagen and the adherence receptors, α1β1 integrin and Staphylococcus aureus Cna MSCRAMM. / Rich, Rebecca L.; Deivanayagam, Champion C S; Owens, Rick T.; Carson, Michael; Höök, Agneta; Moore, Dwight; Yang, Vivian W C; Narayana, Sthanam V L; Höök, Magnus.

In: Journal of Biological Chemistry, Vol. 274, No. 35, 27.08.1999, p. 24906-24913.

Research output: Contribution to journalArticle

Rich, Rebecca L. ; Deivanayagam, Champion C S ; Owens, Rick T. ; Carson, Michael ; Höök, Agneta ; Moore, Dwight ; Yang, Vivian W C ; Narayana, Sthanam V L ; Höök, Magnus. / Trench-shaped binding sites promote multiple classes of interactions between collagen and the adherence receptors, α1β1 integrin and Staphylococcus aureus Cna MSCRAMM. In: Journal of Biological Chemistry. 1999 ; Vol. 274, No. 35. pp. 24906-24913.
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AU - Carson, Michael

AU - Höök, Agneta

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