TREAT Asia Quality Assessment Scheme (TAQAS) to standardize the outcome of HIV genotypic resistance testing in a group of Asian laboratories

Sally Land, Philip Cunningham, Jialun Zhou, Kevin Frost, David Katzenstein, Rami Kantor, Yi Ming Arthur Chen, Shinichi Oka, Allison DeLong, David Sayer, Jeffery Smith, Elizabeth M. Dax, Matthew Law

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32 Citations (Scopus)

Abstract

The TREAT Asia (Therapeutics, Research, Education, and AIDS Training in Asia) Network is building capacity for Human Immunodeficiency Virus Type-1 (HIV-1) drug resistance testing in the region. The objective of the TREAT Asia Quality Assessment Scheme - designated TAQAS - is to standardize HIV-1 genotypic resistance testing (HIV genotyping) among laboratories to permit rigorous comparison of results from different clinics and testing centres. TAQAS has evaluated three panels of HIV-1-positive plasma from clinical material or low-passage, culture supernatant for up to 10 Asian laboratories. Laboratory participants used their standard protocols to perform HIV genotyping. Assessment was in comparison to a target genotype derived from all participants and the reference laboratory's result. Agreement between most participants at the edited nucleotide sequence level was high (>98%). Most participants performed to the reference laboratory standard in detection of drug resistance mutations (DRMs). However, there was variation in the detection of nucleotide mixtures (0-83%) and a significant correlation with the detection of DRMs (p < 0.01). Interpretation of antiretroviral resistance showed ∼70% agreement among participants when different interpretation systems were used but >90% agreement with a common interpretation system, within the Stanford University Drug Resistance Database. Using the principles of external quality assessment and a reference laboratory, TAQAS has demonstrated high quality HIV genotyping results from Asian laboratories.

Original languageEnglish
Pages (from-to)185-193
Number of pages9
JournalJournal of Virological Methods
Volume159
Issue number2
DOIs
Publication statusPublished - Aug 1 2009
Externally publishedYes

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Keywords

  • Drug resistance
  • Genotyping
  • HIV
  • Quality assessment
  • TAQAS

ASJC Scopus subject areas

  • Virology

Cite this

Land, S., Cunningham, P., Zhou, J., Frost, K., Katzenstein, D., Kantor, R., Chen, Y. M. A., Oka, S., DeLong, A., Sayer, D., Smith, J., Dax, E. M., & Law, M. (2009). TREAT Asia Quality Assessment Scheme (TAQAS) to standardize the outcome of HIV genotypic resistance testing in a group of Asian laboratories. Journal of Virological Methods, 159(2), 185-193. https://doi.org/10.1016/j.jviromet.2009.03.016