Transplantation of fetal kidney tissue reduces cerebral infarction induced by middle cerebral artery ligation

Yung Hsiao Chiang, Shinn Zong Lin, Cesario V. Borlongan, Barry J. Hoffer, Marisela Morales, Yun Wang

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

The authors, and others, have recently reported that intracerebral administration of glial cell line-derived neurotrophic factor (GDNF) or osteogenic protein-1 protects against ischemia-induced injury in the cerebral cortex of adult rats. Because these trophic factors are highly expressed in the fetal, but not adult, kidney cortex, the possibility that transplantation of fetal kidney tissue could serve as a cellular reservoir for such molecules and protect against ischemic injury in cerebral cortex was examined. Fetal kidneys obtained from rat embryos at gestational day 16, and adult kidney cortex, were dissected and cut into small pieces. Adult male Sprague-Dawley rats were anesthetized with chloral hydrate and placed in a stereotactic apparatus. Kidney tissues were transplanted into three cortical areas adjacent to the right middle cerebral artery (MCA). Thirty minutes after grafting, the right MCA was transiently ligated for 90 minutes. Twenty-four hours after the onset of reperfusion, animals were evaluated behaviorally. It was found that the stroke animals that received adult kidney transplantation developed motor imbalance. However, animals that received fetal kidney grafts showed significant behavioral improvement. Animals were later sacrificed and brains were removed for triphenyltetrazolium chloride staining, Pax-2 immunostaining, and GDNF mRNA expression. It was noted that transplantation of fetal kidney but not adult kidney tissue greatly reduced the volume of infarction in the cerebral cortex. Fetal kidney grafts showed Pax-2 immunoreactivity and GDNF mRNA in the host cerebral cortex. In contrast, GDNF mRNA expression was not found in the adult kidney grafts. Taken together, our data indicate that fetal kidney transplantation reduces ischemia/reperfusion- induced cortical infarction and behavioral deficits in adult rats, and that such tissue grafts could serve as an unique cellular reservoir for trophic factor application to the brain.

Original languageEnglish
Pages (from-to)1329-1335
Number of pages7
JournalJournal of Cerebral Blood Flow and Metabolism
Volume19
Issue number12
Publication statusPublished - 1999
Externally publishedYes

Fingerprint

Fetal Tissue Transplantation
Middle Cerebral Artery Infarction
Ligation
Glial Cell Line-Derived Neurotrophic Factor
Kidney
Cerebral Cortex
Kidney Transplantation
Transplants
Kidney Cortex
Middle Cerebral Artery
Messenger RNA
Infarction
Reperfusion
Ischemia
Bone Morphogenetic Protein 7
Chloral Hydrate
Wounds and Injuries
Brain
Sprague Dawley Rats
Embryonic Structures

Keywords

  • Glial cell line-derived neurotrophic factor (GDNF)
  • Ischemia
  • Kidney
  • Trophic factors

ASJC Scopus subject areas

  • Endocrinology
  • Neuroscience(all)
  • Endocrinology, Diabetes and Metabolism

Cite this

Transplantation of fetal kidney tissue reduces cerebral infarction induced by middle cerebral artery ligation. / Chiang, Yung Hsiao; Lin, Shinn Zong; Borlongan, Cesario V.; Hoffer, Barry J.; Morales, Marisela; Wang, Yun.

In: Journal of Cerebral Blood Flow and Metabolism, Vol. 19, No. 12, 1999, p. 1329-1335.

Research output: Contribution to journalArticle

Chiang, Yung Hsiao ; Lin, Shinn Zong ; Borlongan, Cesario V. ; Hoffer, Barry J. ; Morales, Marisela ; Wang, Yun. / Transplantation of fetal kidney tissue reduces cerebral infarction induced by middle cerebral artery ligation. In: Journal of Cerebral Blood Flow and Metabolism. 1999 ; Vol. 19, No. 12. pp. 1329-1335.
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