Transplantation of embryonic fibroblasts treated with platelet-rich plasma induces osteogenesis in SAMP8 mice monitored by molecular imaging

Wen Cheng Lo, Jeng Fong Chiou, Juri G. Gelovani, Mei Leng Cheong, Chi-Ming Lee, Hen Yu Liu, Chih Hsiung Wu, Ming Fu Wang, Che Tong Lin, Win Ping Deng

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

The aim of this study was to develop a cell-based boneregeneration approach evaluated by molecular imaging and immunohistochemistry. Methods: Genetically modified NIH3T3 embryonic fibroblasts carrying enhanced green fluorescent protein (NIH3T3-G) were predifferentiated into osteoblastlike cells using platelet-rich plasma (PRP) medium, followed by intraosseous transplantation into ovariectomized senescenceaccelerated mouse prone substrain 8 (OVX-SAMP8 mice). Results: PRP-conditioned NIH3T3-G (PRP/NIH3T3-G) engraftment prevented the development of osteoporosis. Molecular imaging and immunohistochemistry demonstrated the migration of NIH3T3-G cells from the implantation site throughout the skeleton. In situ analyses revealed coexpression of osteopontin and green fluorescent protein in the newly formed bone tissue, demonstrating that the transplant restored the bone trabecular architecture and mineral density in treated OVX-SAMP8 mice. Interestingly, the life span of OVX-SAMP8 mice receiving PRP/NIH3T3-G transplantation was significantly prolonged and similar to that of the congenic senescence-resistant strain of mice. Conclusion: This unique and yet simple approach could potentially be applied to the treatment of senile postmenopausal osteoporosis and perhaps inborn genetic syndromes associated with accelerated aging, such asHutchinson-Gilford progeria syndrome, and for the prolongation of life expectancy in general.

Original languageEnglish
Pages (from-to)765-773
Number of pages9
JournalJournal of Nuclear Medicine
Volume50
Issue number5
DOIs
Publication statusPublished - May 1 2009

    Fingerprint

Keywords

  • Bone
  • Molecular biology
  • Molecular imaging
  • Osteogenesis
  • Platelet-rich plasma
  • Senescence-accelerated mice P8
  • Transplantation

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging

Cite this