Translational upregulation of Aurora-A by hnRNP Q1 contributes to cell proliferation and tumorigenesis in colorectal cancer

Chien Hsien Lai, Yu Chuan Huang, Jenq Chang Lee, Joseph Ta Chien Tseng, Kung Chao Chang, Yen Ju Chen, Nai Jhu Ding, Pao Hsuan Huang, Wen Chang Chang, Bo Wen Lin, Ruo Yu Chen, Yu Chu Wang, Yi Chien Lai, Liang Yi Hung

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

By using RNA-immunoprecipitation assay following next-generation sequencing, a group of cell cycle-related genes targeted by hnRNP Q1 were identified, including Aurora-A kinase. Overexpressed hnRNP Q1 can upregulate Aurora-A protein, but not alter the mRNA level, through enhancing the translational efficiency of Aurora-A mRNA, either in a cap-dependent or -independent manner, by interacting with the 5'-UTR of Aurora-A mRNA through its RNA-binding domains (RBDs) 2 and 3. By ribosomal profiling assay further confirmed the translational regulation of Aurora-A mRNA by hnRNP Q1. Overexpression of hnRNP Q1 promotes cell proliferation and tumor growth. HnRNP Q1/ΔRBD23-truncated mutant, which loses the binding ability and translational regulation of Aurora-A mRNA, has no effect on promoting tumor growth. The expression level of hnRNP Q1 is positively correlated with Aurora-A in colorectal cancer. Taken together, our data indicate that hnRNP Q1 is a novel trans-acting factor that binds to Aurora-A mRNA 5'-UTRs and regulates its translation, which increases cell proliferation and contributes to tumorigenesis in colorectal cancer.

Original languageEnglish
Pages (from-to)e2555
JournalCell death & disease
Volume8
Issue number1
DOIs
Publication statusPublished - Jan 12 2017

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Heterogeneous-Nuclear Ribonucleoproteins
Colorectal Neoplasms
Carcinogenesis
Up-Regulation
Cell Proliferation
Messenger RNA
5' Untranslated Regions
Aurora Kinase A
cdc Genes
Trans-Activators
Growth
Immunoprecipitation
Neoplasms
RNA

ASJC Scopus subject areas

  • Immunology
  • Cellular and Molecular Neuroscience
  • Cell Biology
  • Cancer Research

Cite this

Lai, C. H., Huang, Y. C., Lee, J. C., Tseng, J. T. C., Chang, K. C., Chen, Y. J., ... Hung, L. Y. (2017). Translational upregulation of Aurora-A by hnRNP Q1 contributes to cell proliferation and tumorigenesis in colorectal cancer. Cell death & disease, 8(1), e2555. https://doi.org/10.1038/cddis.2016.479

Translational upregulation of Aurora-A by hnRNP Q1 contributes to cell proliferation and tumorigenesis in colorectal cancer. / Lai, Chien Hsien; Huang, Yu Chuan; Lee, Jenq Chang; Tseng, Joseph Ta Chien; Chang, Kung Chao; Chen, Yen Ju; Ding, Nai Jhu; Huang, Pao Hsuan; Chang, Wen Chang; Lin, Bo Wen; Chen, Ruo Yu; Wang, Yu Chu; Lai, Yi Chien; Hung, Liang Yi.

In: Cell death & disease, Vol. 8, No. 1, 12.01.2017, p. e2555.

Research output: Contribution to journalArticle

Lai, CH, Huang, YC, Lee, JC, Tseng, JTC, Chang, KC, Chen, YJ, Ding, NJ, Huang, PH, Chang, WC, Lin, BW, Chen, RY, Wang, YC, Lai, YC & Hung, LY 2017, 'Translational upregulation of Aurora-A by hnRNP Q1 contributes to cell proliferation and tumorigenesis in colorectal cancer', Cell death & disease, vol. 8, no. 1, pp. e2555. https://doi.org/10.1038/cddis.2016.479
Lai, Chien Hsien ; Huang, Yu Chuan ; Lee, Jenq Chang ; Tseng, Joseph Ta Chien ; Chang, Kung Chao ; Chen, Yen Ju ; Ding, Nai Jhu ; Huang, Pao Hsuan ; Chang, Wen Chang ; Lin, Bo Wen ; Chen, Ruo Yu ; Wang, Yu Chu ; Lai, Yi Chien ; Hung, Liang Yi. / Translational upregulation of Aurora-A by hnRNP Q1 contributes to cell proliferation and tumorigenesis in colorectal cancer. In: Cell death & disease. 2017 ; Vol. 8, No. 1. pp. e2555.
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