Transglutaminase 2 expression is increased as a function of malignancy grade and negatively regulates cell growth in meningioma

Yin Cheng Huang, Kuo Chen Wei, Chen Nen Chang, Pin Yuan Chen, Peng Wei Hsu, Carl P. Chen, Chin Song Lu, Hung Li Wang, David H. Gutmann, Tu Hsueh Yeh

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Most meningiomas are benign, but some clinical-aggressive tumors exhibit brain invasion and cannot be resected without significant complications. To identify molecular markers for these clinically-aggressive meningiomas, we performed microarray analyses on 24 primary cultures from 21 meningiomas and 3 arachnoid membranes. Using this approach, increased transglutaminase 2 (TGM2) expression was observed, which was subsequently validated in an independent set of 82 meningiomas by immunohistochemistry. Importantly, the TGM2 expression level was associated with increasing WHO malignancy grade as well as meningioma recurrence. Inhibition of TGM2 function by siRNA or cystamine induced meningioma cell death, which was associated with reduced AKT phosphorylation and caspase-3 activation. Collectively, these findings suggest that TGM2 expression increases as a function of malignancy grade and tumor recurrence and that inhibition of TGM2 reduces meningioma cell growth.

Original languageEnglish
Article numbere108228
JournalPLoS One
Volume9
Issue number9
DOIs
Publication statusPublished - Sep 23 2014
Externally publishedYes

Fingerprint

protein-glutamine gamma-glutamyltransferase
Cell growth
Meningioma
cell growth
Growth
Neoplasms
Tumors
Cystamine
Phosphorylation
neoplasms
Cell death
caspase-3
Microarrays
small interfering RNA
Arachnoid
Caspase 3
Small Interfering RNA
Recurrence
immunohistochemistry
cell death

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Transglutaminase 2 expression is increased as a function of malignancy grade and negatively regulates cell growth in meningioma. / Huang, Yin Cheng; Wei, Kuo Chen; Chang, Chen Nen; Chen, Pin Yuan; Hsu, Peng Wei; Chen, Carl P.; Lu, Chin Song; Wang, Hung Li; Gutmann, David H.; Yeh, Tu Hsueh.

In: PLoS One, Vol. 9, No. 9, e108228, 23.09.2014.

Research output: Contribution to journalArticle

Huang, YC, Wei, KC, Chang, CN, Chen, PY, Hsu, PW, Chen, CP, Lu, CS, Wang, HL, Gutmann, DH & Yeh, TH 2014, 'Transglutaminase 2 expression is increased as a function of malignancy grade and negatively regulates cell growth in meningioma', PLoS One, vol. 9, no. 9, e108228. https://doi.org/10.1371/journal.pone.0108228
Huang, Yin Cheng ; Wei, Kuo Chen ; Chang, Chen Nen ; Chen, Pin Yuan ; Hsu, Peng Wei ; Chen, Carl P. ; Lu, Chin Song ; Wang, Hung Li ; Gutmann, David H. ; Yeh, Tu Hsueh. / Transglutaminase 2 expression is increased as a function of malignancy grade and negatively regulates cell growth in meningioma. In: PLoS One. 2014 ; Vol. 9, No. 9.
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