Transforming growth factor alpha promotes tumorigenesis and regulates epithelial-mesenchymal transition modulation in colon cancer

Chao Yuan Yu, Wei Chiao Chang, Jia Huei Zheng, Wei Hung Hung, Er Chieh Cho

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Colon cancer is one of the most common cancers in the developed countries. The association between transforming growth factor TGFα and human cancer incidence has been suggested, yet, the regulatory roles of TGFα and the molecular mechanisms remain unknown, especially in colon cancer. We aim to investigate the functional regulations of TGFα in colon cancer progression. Two colon cancer cell lines were applied, and plasmid overexpression and siRNA-mediated depletion techniques were used to verify the role of TGFα in colon cancer. Cell proliferation was analyzed by MTS assay and colony formation assay, and western blot assay was used to examine protein expression. Migration, invasion, and reporter assays were also carried out to study the regulations of TGFα in colon cancer. Our results evidenced that expression of TGFα facilitates short-term and long-term proliferations of colon cancer cells. Moreover, TGFα was suggested as a migration-and-invasion promoting factor of colon cancer. Finally, our data indicated that TGFα modulates epithelial-mesenchymal transition (EMT) markers and NFκB signaling pathway in colon cancer cells. We provide the first time evidence of the promoting role TGFα plays in colon cancer tumorigenesis with proposed regulatory mechanisms involving EMT alteration and NFκB signaling pathway.

Original languageEnglish
Pages (from-to)901-906
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume506
Issue number4
DOIs
Publication statusPublished - Dec 2 2018

Fingerprint

Transforming Growth Factor alpha
Epithelial-Mesenchymal Transition
Colonic Neoplasms
Assays
Carcinogenesis
Modulation
Cells
Cell proliferation
Transforming Growth Factors
Small Interfering RNA
Plasmids
Association reactions
Proteins
Developed Countries
Neoplasms
Western Blotting
Cell Proliferation
Cell Line

Keywords

  • Cell migration and invasion
  • Colon cancer progression
  • Epithelial-mesenchymal transition (EMT)
  • NFκB pathway
  • TGFα

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Transforming growth factor alpha promotes tumorigenesis and regulates epithelial-mesenchymal transition modulation in colon cancer. / Yu, Chao Yuan; Chang, Wei Chiao; Zheng, Jia Huei; Hung, Wei Hung; Cho, Er Chieh.

In: Biochemical and Biophysical Research Communications, Vol. 506, No. 4, 02.12.2018, p. 901-906.

Research output: Contribution to journalArticle

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