Transforming growth factor β1 down-regulates Runx-2 and alkaline phosphatase activity of human dental pulp cells via ALK5/Smad2/3 signaling

Po Shuen Lin, Mei Chi Chang, Chiu Po Chan, Sheng Yang Lee, Jang Jaer Lee, Yi Ling Tsai, Hui Chun Tseng, Tseng Fang Tai, Hsueh Jen Lin, Jiiang Huei Jeng

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Abstract

Objective Transforming growth factor β1 (TGF-β1) plays a role in repair and dentinogenesis in dental pulp. The purpose of this study was to study how TGF-β1 affects 2 differentiation markers, Runt-related transcription factor 2 (Runx-2) and ALP, in dental pulp cells. Study design Primary-cultured human dental pulp cells were treated with TGF-β1 with or without pretreatment and coincubation with 1,4-diamino-2,3-dicyano-1,4-bis(o- aminophenylmercapto)butadiene (U0126, a mitogen-induced extracellular kinase (MEK)/extracellular signal-regulated kinase (ERK) inhibitor), Noggin (a bone morphogenetic protein inhibitor), or 4-(5-benzol[1,3]dioxol-5-yl-4-pyrldin-2-yl- 1H-imidazol-2-yl)-benzamide hydrate (SB431542, an activin receptorlike kinase (ALK) 5/Smad2/3 inhibitor). The differentiation status of pulp cells was evaluated by ALP staining and quantitative ALP activity assay. Changes in ALP and Runx-2 mRNA expression were determined by reverse-transcription polymerase chain reaction. Results Cells under the treatment of TGF-β1 (5 and 10 ng/mL) showed a decrease in ALP activity and gene expression of ALP and Runx-2. Pretreatment by U0126 and Noggin was not effective to prevent the TGF-β1induced decline of ALP activity. Interestingly, SB431542 prevented the TGF-β1induced decline of ALP activity and ALP and Runx-2 gene expression. Conclusion TGF-β1 down-regulates Runx-2 and ALP in human dental pulp cells via ALK5/Smad2/3 signaling. These events may play important roles at specific stages of pulpal repair and dentinogenesis.

Original languageEnglish
Pages (from-to)394-400
Number of pages7
JournalOral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics
Volume111
Issue number3
DOIs
Publication statusPublished - Mar 2011

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ASJC Scopus subject areas

  • Otorhinolaryngology
  • Surgery
  • Dentistry(all)
  • Oral Surgery

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