From the screening of 21 microbial strains, Absidia pseudocylindrospora ATCC 24169 and Aspergillus niger BCRC 32720 were found to reproducibly transform isosteviol lactam (4α-carboxy-13α-amino-13,16-seco-ent-19- norbeyeran-16-oic acid 13,16-lactam) (3) into various compounds. Preparative-scale transformation of 3 with Abs. pseudocylindrospora yielded two new hydroxylated compounds (4 and 5), with conservation of the lactam ring. Preparative-scale transformation of 3 with Asp. niger afforded seven new compounds, 6 and 9 - 14, together with the known compounds 7 and 8. A single-crystal X-ray diffraction experiment confirmed the structure of 14. The suppressive effects of compounds 1 - 14 on the lipopolysaccharide-induced expression of the inducible nitric oxide synthase gene in RAW 264.7 macrophages were examined by a reverse-transcription real-time PCR analysis. With the exception of 7, all other compounds significantly reduced levels of iNOS mRNA relative to control cells, which were induced by LPS alone. Compounds 2, 3, and 5 were similar in activity to dexamethasone, while 9 was more potent.
ASJC Scopus subject areas
- Complementary and alternative medicine
- Molecular Medicine
- Organic Chemistry
- Analytical Chemistry
- Pharmaceutical Science
- Drug Discovery
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Chou, B. (Contributor), Yang, L. (Contributor), Chang, S. (Contributor), Hsu, F. (Contributor), Wang, L. (Contributor), Lin, W. (Contributor), Liu, P. (Contributor) & Lin, S. (Contributor), Unknown Publisher, Jun 24 2011