Transformation of isoflavone phytoestrogens during the fermentation of soymilk with lactic acid bacteria and bifidobacteria

Hsiang Lin Chien, Hui Yu Huang, Cheng Chun Chou

Research output: Contribution to journalArticle

129 Citations (Scopus)

Abstract

In the present study, soymilk is fermented with lactic acid bacteria (Streptococcus thermophilus BCRC 14085, Lactobacillus acidophilus BCRC 14079) and bifidobacteria (Bifidobacterium infantis BCRC 14633, B. longum B6) individually, and in combination. The change in the content of various isoflavones (aglycones, glucoside, acetyl- and malonyl-glucosides) and the β-glucosidase activity in soymilk during fermentation is investigated. It is observed that fermented soymilk contains a lower total isoflavone content (81.94-86.61 μg/ml) than soymilk without fermentation (87.61 μg/ml). Regardless of starter organism employed, fermentation causes a major reduction in the contents of glucoside, malonylglucoside and acetylglucoside isoflavones along with a significant increase of aglycone isoflavones content. The level of change in the content of various isoflavones and β-glucosidase activity after fermentation varies with the starter organism. Among all the fermented soymilks tested, soymilk fermented with S. thermophilus showed the highest β-glucosidase activity and the greatest increase in the contents of aglycones. The percentage of daidzein, genistein and glycitein to total isoflavone content in S. thermophilus-fermented soymilk increases from an initial 14.24%, 6.89% and 2.45%, respectively, to 36.20%, 28.80% and 12.44% after 24 h of fermentation. Finally, the increase of aglycones and decrease of glucoside isoflavones during fermentation coincides with the increase of β-glucosidase activity observed in fermented soymilk.

Original languageEnglish
Pages (from-to)772-778
Number of pages7
JournalFood Microbiology
Volume23
Issue number8
DOIs
Publication statusPublished - Dec 1 2006
Externally publishedYes

Fingerprint

Phytoestrogens
plant estrogens
Bifidobacterium
Isoflavones
soymilk
isoflavones
Fermentation
lactic acid bacteria
Lactic Acid
Glucosidases
fermentation
Bacteria
Glucosides
glucosidases
Streptococcus thermophilus
glucosides
Bifidobacterium longum subsp. infantis
Lactobacillus acidophilus
glycitein
Genistein

Keywords

  • Bifidobacteria
  • Fermented soymilk
  • Isoflavone
  • Lactic acid bacteria

ASJC Scopus subject areas

  • Microbiology
  • Food Science

Cite this

Transformation of isoflavone phytoestrogens during the fermentation of soymilk with lactic acid bacteria and bifidobacteria. / Chien, Hsiang Lin; Huang, Hui Yu; Chou, Cheng Chun.

In: Food Microbiology, Vol. 23, No. 8, 01.12.2006, p. 772-778.

Research output: Contribution to journalArticle

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abstract = "In the present study, soymilk is fermented with lactic acid bacteria (Streptococcus thermophilus BCRC 14085, Lactobacillus acidophilus BCRC 14079) and bifidobacteria (Bifidobacterium infantis BCRC 14633, B. longum B6) individually, and in combination. The change in the content of various isoflavones (aglycones, glucoside, acetyl- and malonyl-glucosides) and the β-glucosidase activity in soymilk during fermentation is investigated. It is observed that fermented soymilk contains a lower total isoflavone content (81.94-86.61 μg/ml) than soymilk without fermentation (87.61 μg/ml). Regardless of starter organism employed, fermentation causes a major reduction in the contents of glucoside, malonylglucoside and acetylglucoside isoflavones along with a significant increase of aglycone isoflavones content. The level of change in the content of various isoflavones and β-glucosidase activity after fermentation varies with the starter organism. Among all the fermented soymilks tested, soymilk fermented with S. thermophilus showed the highest β-glucosidase activity and the greatest increase in the contents of aglycones. The percentage of daidzein, genistein and glycitein to total isoflavone content in S. thermophilus-fermented soymilk increases from an initial 14.24{\%}, 6.89{\%} and 2.45{\%}, respectively, to 36.20{\%}, 28.80{\%} and 12.44{\%} after 24 h of fermentation. Finally, the increase of aglycones and decrease of glucoside isoflavones during fermentation coincides with the increase of β-glucosidase activity observed in fermented soymilk.",
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