Transformation of chicken embryo fibroblasts by direct DNA transfection of single oncogenes: Comparative analyses of src, erbB, myc, and ras

Michael Antczak, Hsing Jien Kung

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Chicken embryo fibroblasts (CEF) have been used extensively to study the transformation parameters of a number of avian sarcoma-leukemia viruses. Previously, oncogene transformation of CEF has been conducted almost exclusively with replicating viruses, because of perceived difficulties with direct DNA transfection. Here, we show that CEF can be efficiently and stably transfected by selection for the neomycin resistance gene (neo). Cotransfection of neo with various oncogenes resulted in CEF transformation in vitro and, In several instances, sarcoma formation in vivo. Transfection of src, myc, erbB, and ras, either singly or in combination, resulted in soft-agar colonies with unique morphologies. Transfection of a family of v-src, c-src, and v/c-src chimeric constructs demonstrated the ability of the assay to discriminate between transforming and nontransforming genes. Transfection of a number of erbB variants showed that internal mutations, primarily in the kinase domain, contribute significantly to the ability to transform fibroblasts. The tumorigenic potential detected by transfection of oncogenes faithfully reproduced those previously reported by using viral infections. Our studies establish the utility of CEF transformation by direct DNA transfection. This method should prove useful in analyzing oncogenes (e.g., myc) that do not readily transform rodent cell lines and in studying host-range mutants of oncogenes, such as those recently identified for src and erbB.

Original languageEnglish
Pages (from-to)1451-1458
Number of pages8
JournalJournal of Virology
Volume64
Issue number4
Publication statusPublished - Dec 1 1990
Externally publishedYes

Fingerprint

oncogenes
transfection
Oncogenes
fibroblasts
Transfection
Chickens
embryo (animal)
Embryonic Structures
Fibroblasts
chickens
DNA
avian sarcoma
Avian Sarcoma Viruses
viruses
myc Genes
Neomycin
neomycin
Host Specificity
sarcoma
Virus Diseases

ASJC Scopus subject areas

  • Immunology

Cite this

Transformation of chicken embryo fibroblasts by direct DNA transfection of single oncogenes : Comparative analyses of src, erbB, myc, and ras. / Antczak, Michael; Kung, Hsing Jien.

In: Journal of Virology, Vol. 64, No. 4, 01.12.1990, p. 1451-1458.

Research output: Contribution to journalArticle

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