Transferred cold atmospheric plasma treatment on melanoma skin cancer cells with/without catalase enzyme in vitro

Yun Hsuan Chen, Jang Hsing Hsieh, I. Te Wang, Pei Ru Jheng, Yi Yen Yeh, Jyh Wei Lee, Nima Bolouki, Er Yuan Chuang

Research output: Contribution to journalArticlepeer-review

Abstract

Cold atmospheric plasma (CAP) is a promising tool to overcome certain cancerous and precancerous conditions in dermatology. A scheme of transferred CAP was first developed to treat melanoma (B16F10) skin cancer cells as well as non-malignant (L929) cells in vitro. CAP was transferred using a silicone tube with a jet system that was developed and was assessed as to whether it could generate reactive oxygen and nitrogen species (RONS) at near-room temperature. The transferred CAP was characterized electrically and spectroscopically. Biological data showed that the transferred CAP killed cancer cells but not non-malignant (L929) cells. Plasma treatment was effective with a time duration of 30 s, whereas non-malignant (L929) cells were less damaged during plasma treatment. In addition, catalase (CAT) enzyme was applied to neutralize and detoxify the RONS generated by the transferred CAP. These findings suggest that transferred CAP can be considered a melanoma cancer therapy.

Original languageEnglish
Article number6181
JournalApplied Sciences (Switzerland)
Volume11
Issue number13
DOIs
Publication statusPublished - Jul 1 2021

Keywords

  • Catalase
  • Cold atmospheric plasma (CAP)
  • Melanoma cell (B16F10)
  • Plasma cancer therapy
  • Reactive nitrogen species (RNS)
  • Reactive oxygen species (ROS)
  • Transferred cold atmospheric plasma

ASJC Scopus subject areas

  • Materials Science(all)
  • Instrumentation
  • Engineering(all)
  • Process Chemistry and Technology
  • Computer Science Applications
  • Fluid Flow and Transfer Processes

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