Transcriptomic analysis of the highly efficient oil-degrading bacterium Acinetobacter venetianus RAG-1 reveals genes important in dodecane uptake and utilization

Ankita Kothari, Marimikel Charrier, Yu Wei Wu, Stephanie Malfatti, Carol E. Zhou, Steven W. Singer, Larry Dugan, Aindrila Mukhopadhyay

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The hydrocarbonoclastic bacterium Acinetobacter venetianus RAG-1 has attracted substantial attention due to its powerful oil-degrading capabilities and its potential to play an important ecological role in the cleanup of alkanes. In this study, we compare the transcriptome of the strain RAG-1 grown in dodecane, the corresponding alkanol (dodecanol), and sodium acetate for the characterization of genes involved in dodecane uptake and utilization. Comparison of the transcriptional responses of RAG-1 grown on dodecane led to the identification of 1074 genes that were differentially expressed relative to sodium acetate. Of these, 622 genes were upregulated when grown in dodecane. The highly upregulated genes were involved in alkane catabolism, along with stress response. Our data suggest AlkMb to be primarily involved in dodecane oxidation. Transcriptional response of RAG-1 grown on dodecane relative to dodecanol also led to the identification of permease, outer membrane protein and thin fimbriae coding genes potentially involved in dodecane uptake. This study provides the first model for key genes involved in alkane uptake and metabolism in A. venetianus RAG-1.

Original languageEnglish
Article numberfnw224
JournalFEMS Microbiology Letters
Volume363
Issue number20
DOIs
Publication statusPublished - 2016

Fingerprint

RAG-1 Genes
Acinetobacter
Oils
Bacteria
Alkanes
Genes
Sodium Acetate
Membrane Transport Proteins
n-dodecane
Transcriptome
Membrane Proteins

Keywords

  • Acinetobacter venetianus RAG-1 ATCC 31012
  • Alkane hydroxylase
  • Alkane monooxygenase
  • Alkane uptake
  • Dodecane
  • Transcriptomic

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Genetics

Cite this

Transcriptomic analysis of the highly efficient oil-degrading bacterium Acinetobacter venetianus RAG-1 reveals genes important in dodecane uptake and utilization. / Kothari, Ankita; Charrier, Marimikel; Wu, Yu Wei; Malfatti, Stephanie; Zhou, Carol E.; Singer, Steven W.; Dugan, Larry; Mukhopadhyay, Aindrila.

In: FEMS Microbiology Letters, Vol. 363, No. 20, fnw224, 2016.

Research output: Contribution to journalArticle

Kothari, Ankita ; Charrier, Marimikel ; Wu, Yu Wei ; Malfatti, Stephanie ; Zhou, Carol E. ; Singer, Steven W. ; Dugan, Larry ; Mukhopadhyay, Aindrila. / Transcriptomic analysis of the highly efficient oil-degrading bacterium Acinetobacter venetianus RAG-1 reveals genes important in dodecane uptake and utilization. In: FEMS Microbiology Letters. 2016 ; Vol. 363, No. 20.
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