Transcriptional regulation of the rat Mrp3 gene promoter by the specificity protein (Sp) family members and CCAAT/enhancer binding proteins

Shwu Jen Tzeng, Wen Chang Chang, Jin Ding Huang

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The sequence of the 5′-flanking region of the rat Mrp3 gene was determined up to 2723 bp upstream of the translation start site. Regulatory regions crucial for Mrp3 promoter activity were characterized between -157 and -106 bp in hepatoma cells. Within this sequence we identified putative binding sites for C/EBP and Sp1. EMSA and supershift assays demonstrated specific binding of Sp1, Sp3, C/EBPα, β, and δ. In Drosophila SL2 cells, both Sp1 and Sp3 transactivated the Mrp3 minimal promoter (pWT-157). Structural and functional analysis demonstrated that binding sites for C/EBPs, Sp1 and Sp3 were essential for transcription of the rat Mrp3 gene in Mrp3-expressing cells (including: H4IIE, H4IIE C3, BRL 3A, Clone 9, and RAT 2). Cotransfection assays demonstrated that C/EBP transcription factors modulated the basal and tissue specific activity of the Mrp3 gene promoter by recognition of the C/EBP (-157/-140) element and through functional cooperation with factors interacting with the Sp1 (3) and Sp1 (4) (-140/-106) cis-acting elements. In this study, we found C/EBPs and Sp1/Sp3 cooperatively regulated the promoter activity of rat Mrp3 gene through proximal (-157/-106) region. It suggested another fine-tune regulation mechanism may involve in Mrp3 gene expression.

Original languageEnglish
Pages (from-to)741-761
Number of pages21
JournalJournal of Biomedical Science
Volume12
Issue number5
DOIs
Publication statusPublished - Oct 2005
Externally publishedYes

Fingerprint

CCAAT-Enhancer-Binding Proteins
Rats
Genes
Assays
Proteins
Binding Sites
Functional analysis
5' Flanking Region
Nucleic Acid Regulatory Sequences
Transcription
Gene expression
Structural analysis
Drosophila
Hepatocellular Carcinoma
Transcription Factors
Clone Cells
Tissue
Gene Expression

Keywords

  • C/EBPα
  • C/EBPβ
  • C/EBPδ
  • Rat Mrp3 regulation
  • Sp1
  • Sp3

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Transcriptional regulation of the rat Mrp3 gene promoter by the specificity protein (Sp) family members and CCAAT/enhancer binding proteins. / Tzeng, Shwu Jen; Chang, Wen Chang; Huang, Jin Ding.

In: Journal of Biomedical Science, Vol. 12, No. 5, 10.2005, p. 741-761.

Research output: Contribution to journalArticle

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