Transcriptional regulation by targeted expression of architectural transcription factor high mobility group A2 in salivary glands of transgenic mice

H. Helen Lin, Ye Xiong, Ye Shih Ho, Beiyun Zhou, Ha Van Nguyen, Hongtao Deng, Renee Lee, Yun Yen, Zea Borok, David K. Ann

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

High mobility group A2 (HMGA2) protein is a non-histone architectural transcription factor. Numerous studies have demonstrated that HMGA2 is exclusively expressed in the nucleus of embryonic, but not of terminally differentiated, cells, and aberrant expression of HMGA2 is associated with various benign tumors, including pleomorphic salivary adenoma. Herein, we report the use of a 4.5-kb enhancer/promoter region of the aquaporin-5 (AQP-5) gene to target HMGA2 transgene expression in the mouse salivary acinar cells as a model to investigate the biochemical and biological role of ectopic HMGA2 expression. The expression pattern was analyzed by microarray analyses to profile HMGA2-dependent salivary gene regulation. By using quantitative reverse transcription-polymerase chain reaction (RT-PCR) assays, the expression of a cluster of genes involved in cytokine signaling, including Il7r, Il2rg, and Ptprc, was verified to be up-regulated in the salivary glands of AQP-5/HMGA2 mice. In concert, the expression of a cluster of genes, namely Ppara, Phyh, and Cidea, governing fatty acid and lipid metabolism, was confirmed to be down-regulated by HMGA2. Additionally, squamous carcinoma-like salivary tumors were observed in the AQP-5/HMGA2 transgenic mice, albeit at a low incidence. Our findings indicate that the AQP-5 promoter/enhancer-containing region is sufficient to target salivary-specific transgene expression and suggest novel roles for HMGA2 in salivary epithelial cells.

Original languageEnglish
Pages (from-to)30-39
Number of pages10
JournalEuropean Journal of Oral Sciences
Volume115
Issue number1
DOIs
Publication statusPublished - Feb 1 2007
Externally publishedYes

Fingerprint

Salivary Glands
varespladib methyl
Transgenic Mice
Transcription Factors
Aquaporin 5
Multigene Family
Transgenes
High Mobility Group Proteins
Pleomorphic Adenoma
Acinar Cells
Microarray Analysis
Lipid Metabolism
Genetic Promoter Regions
Genes
Reverse Transcription
Squamous Cell Carcinoma
Neoplasms
Fatty Acids
Epithelial Cells
Cytokines

Keywords

  • Gene expression
  • HMGA2
  • Microarrays
  • Salivary glands
  • Transgenic mice

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Transcriptional regulation by targeted expression of architectural transcription factor high mobility group A2 in salivary glands of transgenic mice. / Lin, H. Helen; Xiong, Ye; Ho, Ye Shih; Zhou, Beiyun; Nguyen, Ha Van; Deng, Hongtao; Lee, Renee; Yen, Yun; Borok, Zea; Ann, David K.

In: European Journal of Oral Sciences, Vol. 115, No. 1, 01.02.2007, p. 30-39.

Research output: Contribution to journalArticle

Lin, H. Helen ; Xiong, Ye ; Ho, Ye Shih ; Zhou, Beiyun ; Nguyen, Ha Van ; Deng, Hongtao ; Lee, Renee ; Yen, Yun ; Borok, Zea ; Ann, David K. / Transcriptional regulation by targeted expression of architectural transcription factor high mobility group A2 in salivary glands of transgenic mice. In: European Journal of Oral Sciences. 2007 ; Vol. 115, No. 1. pp. 30-39.
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AU - Deng, Hongtao

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