Transcription generates positively and negatively supercoiled domains in the template

Hai Young Wu, Shihua Shyy, James C. Wang, Leroy-Fong Liu

Research output: Contribution to journalArticle

486 Citations (Scopus)

Abstract

We show that transcription of a DNA molecule inside a bacterium is accompanied by local and temporal supercoiling of the DNA template: as transcription proceeds, DNA in front of the transcription ensemble becomes positively supercoiled, and DNA behind the ensemble becomes negatively supercoiled. Because bacterial gyrase and topoisomerase I act differently on positively and negatively supercoiled DNA, the formation of twin supercoiled domains during transcription is manifested by a large increase or decrease in the linking number of an intracellular plasmid when bacterial DNA gyrase or topoisomerase I, respectively, is inhibited. Such changes in linking number are strongly dependent on transcription of the plasmid in cis and on the relative orientations of transcription units on the plasmid. These results indicate that the state of supercoiling of bacterial DNA is strongly modulated by transcription, and that DNA topoisomerases are normally involved in the elongation step of transcription.

Original languageEnglish
Pages (from-to)433-440
Number of pages8
JournalCell
Volume53
Issue number3
DOIs
Publication statusPublished - May 6 1988
Externally publishedYes

Fingerprint

Transcription
Superhelical DNA
Type I DNA Topoisomerase
Plasmids
DNA
DNA Topoisomerases
Bacterial DNA
Bacteria
Elongation
Molecules

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

Transcription generates positively and negatively supercoiled domains in the template. / Wu, Hai Young; Shyy, Shihua; Wang, James C.; Liu, Leroy-Fong.

In: Cell, Vol. 53, No. 3, 06.05.1988, p. 433-440.

Research output: Contribution to journalArticle

Wu, Hai Young ; Shyy, Shihua ; Wang, James C. ; Liu, Leroy-Fong. / Transcription generates positively and negatively supercoiled domains in the template. In: Cell. 1988 ; Vol. 53, No. 3. pp. 433-440.
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