Transcription-driven supercoiling of DNA: Direct biochemical evidence from in vitro studies

Yeou Ping Tsao, Hai Young Wu, Leroy-Fong Liu

Research output: Contribution to journalArticle

239 Citations (Scopus)

Abstract

The translocation of an RNA polymerase elongation complex along double helical DNA has been propose to generate positive supercoiling waves ahead of and negative supercoiling waves behind the transcription ensemble. This twin supercoiled domain model has been tested in vitro. In the presence of prokaryotic DNA topoisomerase I, which selectively removes negative supercoils, transcription from a single promoter results in rapid and extensive positive supercoiling of the DNA template. The accumulation of positive supercoils in the DNA template requires continued movement of the elongation complex as well as sizable nascent RNA chains. These in vitro results provide direct biochemical evidence supporting the twin supercoiled domain model of transcription. Furthermore, the magnitude of DNA supercoiling (torsional) waves generated by transcription is much greater than previously expected, suggesting that transcription is one of the principal affecting intracellular DNA supercoiling.

Original languageEnglish
Pages (from-to)111-118
Number of pages8
JournalCell
Volume56
Issue number1
DOIs
Publication statusPublished - Jan 13 1989
Externally publishedYes

Fingerprint

Transcription
DNA
Elongation
Type I DNA Topoisomerase
Elastic waves
DNA-Directed RNA Polymerases
In Vitro Techniques
RNA

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Medicine(all)

Cite this

Transcription-driven supercoiling of DNA : Direct biochemical evidence from in vitro studies. / Tsao, Yeou Ping; Wu, Hai Young; Liu, Leroy-Fong.

In: Cell, Vol. 56, No. 1, 13.01.1989, p. 111-118.

Research output: Contribution to journalArticle

Tsao, Yeou Ping ; Wu, Hai Young ; Liu, Leroy-Fong. / Transcription-driven supercoiling of DNA : Direct biochemical evidence from in vitro studies. In: Cell. 1989 ; Vol. 56, No. 1. pp. 111-118.
@article{c32a741b94e64c4382d9b74e96551f34,
title = "Transcription-driven supercoiling of DNA: Direct biochemical evidence from in vitro studies",
abstract = "The translocation of an RNA polymerase elongation complex along double helical DNA has been propose to generate positive supercoiling waves ahead of and negative supercoiling waves behind the transcription ensemble. This twin supercoiled domain model has been tested in vitro. In the presence of prokaryotic DNA topoisomerase I, which selectively removes negative supercoils, transcription from a single promoter results in rapid and extensive positive supercoiling of the DNA template. The accumulation of positive supercoils in the DNA template requires continued movement of the elongation complex as well as sizable nascent RNA chains. These in vitro results provide direct biochemical evidence supporting the twin supercoiled domain model of transcription. Furthermore, the magnitude of DNA supercoiling (torsional) waves generated by transcription is much greater than previously expected, suggesting that transcription is one of the principal affecting intracellular DNA supercoiling.",
author = "Tsao, {Yeou Ping} and Wu, {Hai Young} and Leroy-Fong Liu",
year = "1989",
month = "1",
day = "13",
doi = "10.1016/0092-8674(89)90989-6",
language = "English",
volume = "56",
pages = "111--118",
journal = "Cell",
issn = "0092-8674",
publisher = "Cell Press",
number = "1",

}

TY - JOUR

T1 - Transcription-driven supercoiling of DNA

T2 - Direct biochemical evidence from in vitro studies

AU - Tsao, Yeou Ping

AU - Wu, Hai Young

AU - Liu, Leroy-Fong

PY - 1989/1/13

Y1 - 1989/1/13

N2 - The translocation of an RNA polymerase elongation complex along double helical DNA has been propose to generate positive supercoiling waves ahead of and negative supercoiling waves behind the transcription ensemble. This twin supercoiled domain model has been tested in vitro. In the presence of prokaryotic DNA topoisomerase I, which selectively removes negative supercoils, transcription from a single promoter results in rapid and extensive positive supercoiling of the DNA template. The accumulation of positive supercoils in the DNA template requires continued movement of the elongation complex as well as sizable nascent RNA chains. These in vitro results provide direct biochemical evidence supporting the twin supercoiled domain model of transcription. Furthermore, the magnitude of DNA supercoiling (torsional) waves generated by transcription is much greater than previously expected, suggesting that transcription is one of the principal affecting intracellular DNA supercoiling.

AB - The translocation of an RNA polymerase elongation complex along double helical DNA has been propose to generate positive supercoiling waves ahead of and negative supercoiling waves behind the transcription ensemble. This twin supercoiled domain model has been tested in vitro. In the presence of prokaryotic DNA topoisomerase I, which selectively removes negative supercoils, transcription from a single promoter results in rapid and extensive positive supercoiling of the DNA template. The accumulation of positive supercoils in the DNA template requires continued movement of the elongation complex as well as sizable nascent RNA chains. These in vitro results provide direct biochemical evidence supporting the twin supercoiled domain model of transcription. Furthermore, the magnitude of DNA supercoiling (torsional) waves generated by transcription is much greater than previously expected, suggesting that transcription is one of the principal affecting intracellular DNA supercoiling.

UR - http://www.scopus.com/inward/record.url?scp=0024968110&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024968110&partnerID=8YFLogxK

U2 - 10.1016/0092-8674(89)90989-6

DO - 10.1016/0092-8674(89)90989-6

M3 - Article

C2 - 2535966

AN - SCOPUS:0024968110

VL - 56

SP - 111

EP - 118

JO - Cell

JF - Cell

SN - 0092-8674

IS - 1

ER -