Abstract

Traffic emission is responsible for most small-sized particulate matter (PM) air pollution in urban areas. Several recent studies have indicated that traffic-related PM may aggravate kidney disease. Furthermore, exposure to particulate air pollution may be related to the risk of chronic kidney disease (CKD). However, the underlying molecular mechanisms have not been adequately addressed. In the present study, we studied the mechanisms of renal damage that might be associated with exposure to PM. In a real world of whole-body exposure to traffic-related PM model for 3–6 months, PM in urban ambient air can affect kidney function and induce autophagy, endoplasmic reticulum (ER) stress and apoptosis in kidney tissues. Exposure to traffic-related diesel particulate matter (DPM) led to a reduction in cell viability in human kidney tubular epithelial cells HK-2. DPM increased mitochondrial reactive oxygen species (ROS) and decreased the mitochondrial membrane potential. Furthermore, DPM induced ER stress and activated the unfolded protein response (UPR) pathway. Eventually, DPM exposure induced caspase pathways and triggered apoptosis. In addition, DPM induced autophagy through the inhibition of the Akt/mTOR pathway. Autophagy inhibition resulted in significantly increased cytotoxicity and apoptosis. These findings suggest that air pollution in urban areas may cause nephrotoxicity and autophagy as a protective role in PM-induced cytotoxicity.

Original languageEnglish
Pages (from-to)235-244
Number of pages10
JournalFree Radical Biology and Medicine
Volume135
DOIs
Publication statusPublished - May 1 2019

Fingerprint

Particulate Matter
Autophagy
Air Pollution
Air pollution
Kidney
Endoplasmic Reticulum Stress
Cytotoxicity
Apoptosis
In Vitro Techniques
Unfolded Protein Response
Mitochondrial Membrane Potential
Kidney Diseases
Caspases
Chronic Renal Insufficiency
Reactive Oxygen Species
Cell Survival
Epithelial Cells
Air
Cells
Tissue

Keywords

  • Apoptosis
  • Autophagy
  • Endoplasmic reticulum stress
  • Nephrotoxicity

ASJC Scopus subject areas

  • Biochemistry
  • Physiology (medical)

Cite this

Traffic-related particulate matter exposure induces nephrotoxicity in vitro and in vivo. / Hsu, Yung Ho; Chuang, Hsiao Chi; Lee, Yu Hsuan; Lin, Yuh Feng; Chen, Yi Jie; Hsiao, Ta Chih; Wu, Mei Yi; Chiu, Hui Wen.

In: Free Radical Biology and Medicine, Vol. 135, 01.05.2019, p. 235-244.

Research output: Contribution to journalArticle

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