Tracking the rejection and survival of mouse ovarian iso- and allografts in vivo with bioluminescent imaging

Chi Huang Chen, Yu Chi Yeh, Gwo Jang Wu, Yen Hua Huang, Wen-FuThomas Lai, Jah Yao Liu, Chii Ruey Tzeng

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The applications of in vivo bioluminescent imaging (BLI) with a luciferase reporter gene occur widely across biomedical fields. Luciferasetransgenic mice are highly useful donors for tracking transplanted ovarian tissues. Realizing the full potential of this system may greatly benefit the study of the physiological behaviour and function of transplanted grafts, and the rapid and reliable evaluation of new transplantation protocols. The ovarian tissues of donor FVB/N-Tg(PolII-Luc)Ltc transgenic mice, with a luciferase transgene as the reporter, were transplanted into iso/allogeneic recipients. Rejection, ovarian function and BLI were quantitatively analysed in vivo over time. The BLI of the ovarian isografts revealed longer survival than that of allografts, even with cyclosporine A (CsA) treatment. The CD4+/CD8+ratios of peripheral T-cells were significantly reduced in allografts compared with those in isografts (P+ cell numbers were higher in allografts. The infiltration of CD4+/CD8 + cells into the graftwas unremarkable in isografts from day 1, butwas strong in allografts from day 8 onwards. Hormone activity revealed complete oestrus cycles in the isografts but only the dioestrus stage in the allografts. These results demonstrate that BLI in vivo expedites the fast throughput and fate maps of ovarian grafts. The use of BLI to longitudinally monitor ovarian grafts for immunorejection demonstrated the short survival of allografts and the much longer survival of isografts. CsA treatment alone is ineffective against the acute rejection of ovarian allografts.

Original languageEnglish
Pages (from-to)105-112
Number of pages8
JournalReproduction
Volume140
Issue number1
DOIs
Publication statusPublished - 2010

Fingerprint

Isografts
Allografts
Luciferases
Transplants
Cyclosporine
Diestrus
CD4-CD8 Ratio
Estrus
Rejection (Psychology)
Transgenes
Reporter Genes
Transgenic Mice
Cell Count
Transplantation
Tissue Donors
Hormones
T-Lymphocytes

ASJC Scopus subject areas

  • Obstetrics and Gynaecology
  • Reproductive Medicine
  • Embryology
  • Endocrinology
  • Cell Biology
  • Medicine(all)

Cite this

Tracking the rejection and survival of mouse ovarian iso- and allografts in vivo with bioluminescent imaging. / Chen, Chi Huang; Yeh, Yu Chi; Wu, Gwo Jang; Huang, Yen Hua; Lai, Wen-FuThomas; Liu, Jah Yao; Tzeng, Chii Ruey.

In: Reproduction, Vol. 140, No. 1, 2010, p. 105-112.

Research output: Contribution to journalArticle

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